Organ-on-a-Chip - Complete Setup
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Organ-on-a-Chip - Complete Setup

This modular Organ-on-a-Chip platform represents an innovative approach to create 3D cell cultures and tissues with a precise and continuous control of culturing parameters. It is possible to mimic tissue organization, cell-cell interactions, extracellular matrix influence and physiological responses in a more in vivo-like environment. The inlets and outlets with integrated Luer connections ensure leak-free junctions with the tubing and allow experiments under dynamic flow conditions.
The setup includes Microfluidic Chips, a Clamp and Culture Patches! All you need to create your 3D cell culture.
Available within 2-3 weeks
€400.00
This modular Organ-on-a-Chip platform represents an innovative approach to create 3D cell cultures and tissues with a precise and continuous control of culturing parameters. It is possible to mimic tissue organization, cell-cell interactions, extracellular matrix influence and physiological responses in a more in vivo-like environment. The inlets and outlets with integrated Luer connections ensure leak-free junctions with the tubing and allow experiments under dynamic flow conditions.
The setup includes Microfluidic Chips, a Clamp and Culture Patches! All you need to create your 3D cell culture.

Description

An ideal Organ-on-a-Chip should be easy to use and with a reversible access to cells in chip for downstream analysis. Hence our Organ-on-Chip meets all these demands. Available in Small pack (1 chip - 1 clamp - 4 patches) or Large pack (4 chips - 1 clamp - 16 patches).

Three components characterize this microfluidic platform:

  • Microfluidic chip
  • Clamp
  • Culture patch

The modular microfluidic chip consists in two plastic plates in PC (Polycarbonate), each of these has four integrated magnets for reversible assembly and an elastomeric PDMS thin film to ensure good sealing up to pressures of 8 kPa. The Luer Connectors in the upper part of the chip allow medium perfusion and dynamic flow conditions during your experiments of drug screening and/or cell differentiation.

The clamp, included in the pack, ensures good sealing with high flow pressures (up to 100 kPa). The chip can be placed in a hand screwed clamp for assembling and fast operation and, at the end of your experiment in dynamic flow conditions, the tissue patch can be easily removed by disassembling the clamps and the microfluidic chip. 

The culture patch is a monolayer of nanofibers and support the organization of cells in a functional 3D microtissue. Culture patches are included in the setup and nanofibers can be made of two different natural polymers to mimic the extracellular matrix:

  • gelatin
  • collagen
Please, contact us if you want a custom culture patch made of synthetic biocompatible polymers:
  • PLGA poly(lactic-co-glycolic acid)
  • PCL polycaprolactone
  • PMGI polydimethylglutarimide

Thus, different patches can be used to culture cells or to differentiate Human Induced Pluripotent Stem Cells (hiPSC) in dish or microplates in order to create your tissue patch (e.g. cardiac, neuron, etc). Then, the obtained tissue patch (or other tissue culture inserts) can be integrated in the assembled Organ-on-Chip. A co-culture of two different cell types is possible as well. In order to seed the cells on both sides a coating of matrigel and collagen is needed. The ratio of the mix will be cell type dependent. 

Click here if you want to know more about the available culture patches!

Setup

Once the assembly is completed, micro-chambers and micro-channels are patterned in both plastic plates of the chip to create two independent microfluidic systems: the upper and the lower chambers separated by the tissue patch. These two microfluidic chambers can be perfused with different culture mediaThus a tissue interface can be formed to simulate alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc. Tissues inside the chip can be observed by microscopy or harvested for downstream analysis. 

 

 

 

Microfluidic chip 

Upper plate size 55 mm x 25 mm x 8 mm
Lower plate size 55 mm x 25 mm x 3 mm
Dimension for patch insert Φ 13 X 0.2 mm²
Channel width 1000 μm
Chamber depth 500 μm
Material PC with PDMS thin layer
Connection Luer connectors

 

Culture patch

Materials Natural or Synthetic biocompatible polymers 
Dimensions Φ 13 X 0.2 mm² 
Nanofiber diameter 100-500 nm
Nanofiber thickness < 1 µm
Porosity 60-80 %


Clamp

Dimension  80 mm x 25 mm x 3 mm
Number of windows 1
Window size 40 mm x 20 mm x 3 mm
Material  Hard aluminum

The Small Pack setup includes:

  • 1x Microfluidic Chip with four Luer Connectors (Upper plate + Lower plate)
  • 1x Clamp
  • 4x Culture Patches

The Large Pack setup includes:

  • 4x Microfluidic Chips with four Luer Connectors (Upper plate + Lower plate)
  • 1x Clamp
  • 16x Culture Patches

 

We offer different Culture Patches, choose it accordingly to your cell culture and create your own 3D tissue.

The complete setup comes sterile and have to be stored in dry conditions, without direct exposition to the sunlight at room temperature (15-25 °C).   

 

Click here if you want to order Microfluidic Chip, Culture Patches and Clamp separately!

 

Integration of a tissue patch into the chip gives rise to Organ-on-Chip models. Typically, a tissue patch made of two types of cells can be inserted in the interface of two elastoplastic plates. After clamping, solutions can injected to create physiological conditions or test the effects of drugs and growth factors.  

Benefits:

  • Reversible patch integration thanks to the modular chip
  • Leak-free Luer Connection molded with the chip 
  • Highly-resistant and optically clear material
  • Standard microscope glass slide size

 

By changing the cell type and the patch, this chip can be used to create:

  • Neural or cardio-vascular networks-on-a-chip,
  • Gut-on-a-chip,
  • Liver-on-a-chip,
  • Skin-on-a-chip,
  • Lung-on-a-chip,
  • Mesenchymal stem cells (MSC) or bone marrow-on-a-chip,
  • ESC or iPSC-derived stem cells (ESC/iPSC)-on-a-chip.
A significative example of application: Lung Alveolus modeling. By differentiating hiPSCs into epithelial and endothelial cells on upper and lower sides of a culture patch, an alveolar tissue is obtained and can be integrated into the microfluidic chip to recapitulate the pulmonary physiological conditions. By injecting growth factors, nano-materials or drugs, it is possible to study the extravasation and translocation of the tested compounds at the alveolar epithelium level.

    This modular microfluidic platform significantly improve the predictability of drug screening and studies of personalized medicine.  

    Useful cell culture protocols can be find in these papers published in high impact factor journals:

    • Tang et al., Effective motor neuron differentiation of hiPSCs on a patch made of crosslinked monolayer gelatin nanofibers. J. Mater. Chem. B, 2016, 4, 3305 (PDF file)

    Figure 1. Neuronal cells in red ( Neuron-specific class III beta-tubulin TUJ1 positive) and Astrocytes in green (glial fibrillary acidic protein GFAP positive)

     

     

    • Tang et al., Induction and differentiation of human induced pluripotent stem cells into functional cardiomyocytes on a compartmented monolayer of gelatin nanofibers. Nanoscale, 2016, 8, 14530 (PDF file)
    Figure 2. On patch cardiac differentiation of hiPSCs. (A) Schematic of cardiac differentiation under off-ground conditions. (B) Protocol of cardiac differentiation. (C) Bright-field images of the differentiating hiPSCs at day −1, 1, 3, 5 and 12.

     

    User guide

     

    • Performing an experiment under dynamic flow conditions is possible in many different ways, it depends on the type of results you aim to obtain:
      1. If your experiment requires a short exposure time you can perform it without incubator by just keeping the medium at the right temperature and using CO2 independent medium (Leibovitz L-15 for example);
      2. If your experiment requires a prolonged incubation you can put the chip in the incubator and leave the perfusion system outside; the use of thin tubes (1/16" OD as an example) can facilitate the task because they can pass through the incubator's door without perturbing the correct closure.
    • The sterilization of the components is possible by using a solution of 70% ethanol or by UV exposition (under the UV light of the hood for example).
    • It would be possible to reuse the chip. However, we recommend to not use the chip with different cells in order to avoid any possible cross contamination. In our test, we use the same chip often with different patches of the same cells in culture but with some limitations: the chip has some PDMS parts inside and the prolonged use could increase the absorption of chemicals with possible toxic effects for the cells. The chip should be reused 3-4 times but this will strongly depend on your specific working conditions. 
    • You can remove bubbles from the device by priming it with culture medium. It is possible to inject the medium in the empty device with increasing pressures until bubbles are flushed away. We recommend to perform this process before to seed the cells. This should be easy if you seed the cells on the support once the device is assembled.
      On the other hand, if you seed the cells on the support and then put it in the device, you can try to inject the media once the device is assembled. We suggest to start with a very low pressure and then to increase slowly the pressure and verify that the cells are not detaching from the support.

    Payment Terms

    You can either order and pay via credit card or invoice (through a purchase order). Please note that some products are not available for direct purchase: just click "Request a Quote" on the product page and fill in the form to get more informations!

    Credit card 

    • Add your products to the cart
    • In the cart page, click on Proceed to Checkout
    • Complete your credit card informations (don't worry, we are using Stripe, a PCI-certified program specialized in online transactions)
    • That's it! You will receive an order confirmation within a few minutes, and our team will send you the invoice.

    Invoice

    • Add your products to the cart
    • In the website header, click on Request a Quote
    • Fill in the required informations, then click on Submit Quote
    • Our team will send you a quote shortly
    • After receiving the quote, just send your purchase order at contact@darwin-microfluidics.com
    • Our team will complete your purchase and send you the invoice!

    If you wish to pay in USD, AUD or GBP, please specify it in your PO. We can add the converted value in the required currency to the quote if requested.

     

    Shipping

    The shipping charges are calculated based on the weight of the package and on the country of destination. We try to offer you the best shipping rates and services, shipping all our orders through DHL Express. Generally, your order reaches your lab in only 2 to 3 days after we send it, no matter the destination! 

    Please note that our shipping incoterms are DAP (Delivered At Place). Please contact us if you prefer using your own courrier account or have any question!