Organ-on-a-Chip - LiveBox1
- Product SKU: IV-K01-kit LB1-EU
- Category: Cell Culture, IVTech, Microfluidic Chips, Microfluidic Organ-on-a-chip
LB1 is an Organ-on-a-Chip platform characterized by a transparent bioreactor to host 2D or 3D cell cultures and tissues (e.g. biopsies). The mono-chamber of this chip is designed to ensure an in vivo-like environment under static or dynamic flow conditions thanks to the presence of flow inlet and outlet for the perfusion of culture media and testing solutions.
The setup includes all you need to perform your experiment: a LB1 bioreactor with its Clamp, Tubes, Connectors and a Mixing Chamber for media and reagents.
LiveBox1 is an innovative bioreactor developed by IVTech, an italian biotech company whose aim is to provide a new kind of in vitro models based on dynamic fluid flows. Darwin Microfluidics as partner of IVTech offers the same purchase conditions together with a scientific support in 3D cell culture and microfluidics.
This mono-chamber device allows a precise and continuous control of culturing parameters. It is possible to mimic tissue organization, cell-cell interactions and physiological responses to external stimuli by maintaining the sample in a dynamic environment.
A standard microscope Glass Disc Coverslip (Φ 20 mm and thickness 0.17 mm) represents the support of your cell culture and can be 1) easily placed into the chamber and 2) removed from it at the end of your experiment. The Glass Disc Coverslip is optically transparent and make the bioreactor suitable for microscopic imaging.
The integrated inlet and outlet together with the clamp system ensure leak-free junctions in both static and dynamic experiments (flow rate up to 1 mL/min).
All the components of the cell culture chamber are made in medical silicone PE500, so the chamber can be used several times (you have just to replace the glass coverslip) and tolerates different washing and sterilization cycles in autoclave.
Features & Advantages
The Organ-on-a-Chip LB1 is designed to offer a reversible access to the cultured cells and tissues.
The bioreactor has the same volume of the single well of a 24-well plate to allow you to use your standard protocols, cell dilutions and common cell culture settings.
The bottom glass support makes the microscopic imaging convenient:
Real-time live imagining of the 2D/3D cell culture during the static or dynamic experiments by using bright-field, fluorescence or confocal microscopes.
Down-stream staining and imaging analysis; since the glass support is removable, you have the possibility to disassemble the chamber and remove the glass disc with the tissue! Use it for further investigations such as high-resolution imaging.
The interconnection of different bioreactors can create a modular system to mimic the cross-talk between different tissues in a Body-on-a-Chip configuration.
Components of the platform:
- LiveBox1 bioreactor equipped with glass discs
- Silicon tubes with Luer Lock connectors
- Mixing chamber / reservoir with its support
- Extractor to easily remove the glass disc
The culture chamber, the bottom glass disc and the clamp, once assembled together, create an optically transparent bioreactor with a perfect sealing up to flow rates of 0.4 mL/min. The inlet and outlet allow medium perfusion and dynamic flow conditions during your experiments of drug screening and/or cell differentiation.
LB1 is compatible with commercial peristaltic pumps (click here to see our large offer!), syringe pumps (click here to see our large offer!) and with the innovative LiveFlow Pump that can be placed directly in the wet environment of your cell incubator (click here for more information!).
1x LB1 bioreactor
|Material||Medical grade silicone PE500|
|Cell culture support||2x Glass Disc Coverslip (Φ 20 mm and thickness 0.17 mm)|
|Chamber Volume||1.5 mL|
|Inlet/Outlet||2x Luer Locks M/F (polypropylene)|
|Suggested Flow Rate||0.1 to 0.4 mL/min|
1x Clamp System
|Material||Derlin and Acrylonitrile butadiene styrene (ABS)|
|Window size||Φ 20 mm|
1x Mixing Chamber
|Material||ABS; Stainless steel spine|
|Component||A support to safely remove the cell culture chamber from the clamp system at the end of the experiment; it is to avoid the break the Glass Disc|
|Material||Medical Silicone PE500|
The individual components are available upon request.
The complete setup comes not sterile and have to be stored in dry conditions, without direct exposition to the sunlight at room temperature (15-25 °C). You can sterilize it by autoclave or gasplasma.
IVTech technology is based on compact, user-friendly and transparent cell culture chambers with shape and dimensions similar to the classical 24 well plate (1.5 mL/culture chamber), widely used in biological research. This allow you to use the same standard protocols of your habitual cell culture experiments with the advantages to create 3D tissues and a dynamic environment.
The design and dimensions of the LiveBox1 make it suitable for the direct creation of a 3D complex tissue through the use of a 3D bioprinter!
Seed your cell line on the standard glass disc and, once the assembly is completed, the mono-chamber is hermetically closed and ready to work in dynamic conditions. After clamping, solutions can be injected to recreate the physiological conditions or test the effects of drugs and growth factors.
Tissues inside the bioreactor can be observed by microscopy or harvested for downstream analysis.
- Reversible glass cosverslip integration thanks to the modular bioreactor
- Leak-free Luer Connections
- Highly-resistant and optically clear materials
- Dimensions compatibile with microscopes observation
Examples of application
This modular platform significantly improve the predictability of drug screening and studies of personalized medicine.
LiveBox1 has been successfully used to create the following tissues:
- Physiological models: Liver, Muscle
- Disease models: Glaucoma, Breast Cancer
- Multi-organ models: Obesity
Useful cell culture protocols can be find in these papers published in high impact factor journals:
Case study n° 1: Biopsy (muscle explant)
Figure 1. Soleus muscle, samples maintained in culture under fluid dynamic (a,c,e) or conventional (b,d,f ) conditions. After 24 h incubation in the bioreactor (c), cytoskeletal organization is still recognizable, and mitochondria (arrows), sarcoplasmic reticulum (arrowheads) and glycogen (thin arrows) are well preserved; under conventional condition (d) the cytoskeletal architecture is loosened, mitochondria (arrows) and sarcoplasmic reticulum (arrowheads) are swollen and glycogen is lost. After 48 h in the bioreactor (e), sarcomere organization is hardly recognizable, sarcoplasmic reticulum (arrowheads) is swollen, but mitochondria (arrows) are still preserved; under conventional condition (f ), the cytoskeletal organization is definitely lost, and mitochondria (arrows) and sarcoplasmic reticulum (arrowheads) are strongly damaged. Scale bars: 1000 nm. Insets scale bars: 10 μm.
Case study n° 2: Breast Cancer Model (drug cytotoxicity)
Figure 2. A 3D culture of MCF7 is more resistant than 2D culture to the treatment with Trastuzumab. However, both culture conditions show a higher sensitivity when the drug test is performed in a dynamic environment. The fluid flow provide a prediction closer to the in vivo drug efficacy than the static models.
Case study n° 3: Obesity Model (multi-organs)
Figure 3. a) Basic components of the 3-way fluidic system with LB1 and LB2 chambers; b) fluidic setup with peristaltic pump, mixing chamber and hepatic (LB1), endothelial and adipose tissue (LB2) chambers; c) HepG2 cells stained with DAPI (blue) and actin-phalloidin (red) seeded on porous collagen scaffolds (green autofluoresence). Scale bar 50 μm; d) endothelial cells stained with calcein. Scale bar 50 μm; e) adipocytes stained with oil-red. Scale bar 50 μm.
- Performing an experiment under dynamic flow conditions is possible in many different ways, it depends on the type of results you aim to obtain:
- If your experiment requires a short exposure time you can perform it without incubator by just keeping the medium at the right temperature and using CO2 independent medium (Leibovitz L-15 for example);
- If your experiment requires a prolonged incubation you can use the innovative LiveFlow Pump that can be placed directly in the wet environment of your cell incubator together with the bioreactor (click here for more information!).
- The sterilization of the components is possible trough an autoclave or by using a gasplasma.
- It would be possible to reuse the chip after washing and sterilization. Only the Glass Discs have to be changed after each utilization.
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