Active Droplet Sorting Chip - PDMS
This sorting chip is designed for the high-throughput analysis and sorting of single cells in droplets. Droplets are first re-injected in one of the inlets and spaced by a flow focusing design. It is followed by an active sorting site with electrodes, to be used with a high-voltage amplifier.
3 devices per chip, with 0.5 mm or 1.2 mm punched holes to accommodate any size of tubing.
General
This droplet sorting chip in PDMS combines a standard flow focusing design for the re-injection and spacing of the droplets, and an active droplet sorter. Droplets should be generated in a first chip before being re-injected.
Electrical connection from the sorting site to a high-voltage amplifier is made with ITO electrodes and couplers.
Fluidic channels are coated with a silane mix after bonding to guarantee good and consistent hydrophobic properties.
Choose the punched hole size according to your requirements:
- 0.5 mm: for 1/32" OD rigid tubing (PTFE, PEEK, FEP, PFA, etc.) or 23G couplers for soft-walled tubing
- 1.2 mm: for 1/16" OD rigid tubing (PTFE, PEEK, FEP, PFA, etc.) or 18G couplers for soft-walled tubing
Content
1x Active droplet sorting chip in PDMS
Specifications
| DG-SRT-ITO-05 | DG-SRT-ITO-12 | |
| Nozzle width | 25 µm | 25 µm |
| Collection channel width | 80/120 µm | 80/120 µm |
| Channel height | 30 µm | 30 µm |
| Material | PDMS | PDMS |
| Dimensions | standard glass slide (75 x 25 mm) | standard glass slide (75 x 25 mm) |
| Number of devices per chip | 3 | 3 |
| Number of inlets | 2 | 2 |
| Number of outlets | 2 | 2 |
| Punched hole size | 0.5 mm | 1.2 mm |
| For rigid tubing OD | 1/32" | 1/16" |
| Couplers for soft-walled tubing | 23G | 18G |
Documentation
Mazutis, L., Gilbert, J.R., Ung, W.L., Weitz, D.A., Griffiths, A.D., & Heyman, J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature Protocols, 8, 870-891. https://doi.org/10.1038/nprot.2013.046
This sorting chip is designed for the high-throughput analysis and sorting of single cells in droplets. Droplets are first re-injected in one of the inlets and spaced by a flow focusing design. It is followed by an active sorting site with electrodes, to be used with a high-voltage amplifier.
3 devices per chip, with 0.5 mm or 1.2 mm punched holes to accommodate any size of tubing.
General
This droplet sorting chip in PDMS combines a standard flow focusing design for the re-injection and spacing of the droplets, and an active droplet sorter. Droplets should be generated in a first chip before being re-injected.
Electrical connection from the sorting site to a high-voltage amplifier is made with ITO electrodes and couplers.
Fluidic channels are coated with a silane mix after bonding to guarantee good and consistent hydrophobic properties.
Choose the punched hole size according to your requirements:
- 0.5 mm: for 1/32" OD rigid tubing (PTFE, PEEK, FEP, PFA, etc.) or 23G couplers for soft-walled tubing
- 1.2 mm: for 1/16" OD rigid tubing (PTFE, PEEK, FEP, PFA, etc.) or 18G couplers for soft-walled tubing
Content
1x Active droplet sorting chip in PDMS
Specifications
| DG-SRT-ITO-05 | DG-SRT-ITO-12 | |
| Nozzle width | 25 µm | 25 µm |
| Collection channel width | 80/120 µm | 80/120 µm |
| Channel height | 30 µm | 30 µm |
| Material | PDMS | PDMS |
| Dimensions | standard glass slide (75 x 25 mm) | standard glass slide (75 x 25 mm) |
| Number of devices per chip | 3 | 3 |
| Number of inlets | 2 | 2 |
| Number of outlets | 2 | 2 |
| Punched hole size | 0.5 mm | 1.2 mm |
| For rigid tubing OD | 1/32" | 1/16" |
| Couplers for soft-walled tubing | 23G | 18G |
Documentation
Mazutis, L., Gilbert, J.R., Ung, W.L., Weitz, D.A., Griffiths, A.D., & Heyman, J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature Protocols, 8, 870-891. https://doi.org/10.1038/nprot.2013.046