Organ-on-a-Chip - Tissue & Endothelial Barrier
Organ-on-a-Chip - Tissue & Endothelial Barrier
Organ-on-a-Chip - Tissue & Endothelial Barrier
Organ-on-a-Chip - Tissue & Endothelial Barrier
Organ-on-a-Chip - Tissue & Endothelial Barrier
Organ-on-a-Chip - Tissue & Endothelial Barrier
Organ-on-a-Chip - Tissue & Endothelial Barrier
Organ-on-a-Chip - Tissue & Endothelial Barrier
Organ-on-a-Chip - Tissue & Endothelial Barrier
Organ-on-a-Chip - Tissue & Endothelial Barrier
Organ-on-a-Chip - Tissue & Endothelial Barrier
Organ-on-a-Chip - Tissue & Endothelial Barrier

Organ-on-a-Chip - Tissue & Endothelial Barrier

Available: In Stock
This Organ-on-a-Chip with central chamber and flanked microchannels allows to create 3D tissue models that accelerate real-time studies of cellular interactions, extravasation and drug delivery. It provides a morphologically and biologically realistic microenvironment that more accurately depicts the in vivo reality. The inlets and outlets are directly compatible with 1/16" OD tubing for the introduction of cells and reagents. Performing experiments under dynamic flow conditions is possible as well.
Add Tubing Clamps and 24G Needles to start using the setup easily out of the box!
€105.00
This Organ-on-a-Chip with central chamber and flanked microchannels allows to create 3D tissue models that accelerate real-time studies of cellular interactions, extravasation and drug delivery. It provides a morphologically and biologically realistic microenvironment that more accurately depicts the in vivo reality. The inlets and outlets are directly compatible with 1/16" OD tubing for the introduction of cells and reagents. Performing experiments under dynamic flow conditions is possible as well.
Add Tubing Clamps and 24G Needles to start using the setup easily out of the box!

 

Description

The design of this chip allows to mimic 1) the formation of 3D tissues and endothelial barrier and 2) their cross-talk interactions. The device includes slits or gaps to form the intersection between the outer channel and inner chamber. In this way it is possible to recreate tight and gap junctions essential for a reliable tissue modelling (such as the blood-brain barrier and other endothelial/tissue interfaces). 

The possibility to recreate 3D tissue models accelerates real-time studies of cellular behavior and drug screening by providing a biological and morphological microenvironment that more accurately depicts in vivo reality and ensuring a convenient real-time visualization.

Several options for channel size, tissue chamber size, scaffolding and barrier design are available (see the Specifications). We can support you in your choice and select the right parameters by following your needs. 

This smart and innovative design overcomes the current limitations inherent in flow chambers or Transwell chamber based assays. Current flow chamber designs are oversimplified, lack the scale and geometry of the microenvironment and cannot model transmigration. Similarly, Transwell chambers do not account for fluid shear and size/topology observed in vivo, the end point measurements of migration are not reproducible and do not provide real-time visualization.

This simplified and idealized microvascular network easily reproduce the cellular stratification, constant shear and flow conditions.

 

Benefits:

  • Side by side architecture enables quantitative real time visualization
  • Physiological leaky vasculature with engineered porous structures
  • Physiologically realistic convective and diffusive transport
  • Microfluidic platform with ultra-low consumable volumes
  • Highly-resistant and optically clear material
  • Standard microscope glass slide size

 

Options (see available designs and options details in the "Specifications" tab):

    • Design 1
      • Option A
        • 1) 100µm slit spacing - 2µm wide slit - 50µm barrier width
        • 2) 50µm slit spacing - 3µm wide slit - 50µm barrier width
        • 3) 50µm slit spacing - 3µm wide slit - 100µm barrier width
        • 4) 50µm slit spacing - 2µm wide slit - 50µm barrier width
        • 5) 50µm slit spacing - 2µm wide slit - 100µm barrier width
      • Option B
        • 1) 3µm pillar diameter - 3µm gap - 100µm barrier width
        • 2) 3µm pillar diameter - 8µm gap - 50µm barrier width
        • 3) 3µm pillar diameter - 3µm gap - 50µm barrier width
        • 4) 3µm pillar diameter - 8µm gap - 100µm barrier width
        • 5) 8µm pillar diameter - 3µm gap - 100µm barrier width
        • 6) 8µm pillar diameter - 8µm gap - 50µm barrier width
    • Design 2
      • Option A
        • 1) 50µm slit spacing - 2µm wide slit - 100µm barrier width
      • Option B
        • 1) 3µm pillar gap
        • 2) 8µm pillar gap

    Create your co-culture and mimic the formation of complex 3D structures such as the blood-brain barrier or other endothelial/tissue interfaces by choosing one of these suitable chip configurations. 

    Idealized co-culture constructs are available with several options for channel size, intersection design, tissue chamber size and scaffolding. We can help you select the right parameters for your needs, just write to contact@darwin-microfluidics.com

     

    Design 1 (co-culture-Radial design)

    The radial design of this configuration has a pattern that consists in a central chamber flanked by two outer channels. The depth of all channels is 100 µm and a barrier region represents the intersection between them and the tissue chamber. The chamber and channels have inlets and outlets directly compatible with 1/16" Tygon tubing.

     

    Option A - Slit Barrier (suitable for metastasis-invasion assay, BBB modeling, liver-toxicology modeling)

    These devices have slits and gaps to form the barrier region between the outer channel and inner chamber.

    Here are the parameters of the available patterns:

    • Outer Channel Width (OC): 100 μm or 200 μm
    • Travel Width (T): 50 μm or 100 μm
    • Slit Spacing (SS): 50 μm or 100 μm
    • Slit Width: 2 μm or 3 µm

     

    Option B - Pillar Barrier (suitable for inflammation modeling)

    These devices have pillars forming the barrier region between the outer channel and inner chamber.

     Here are the parameters of the available patterns:

    • Outer Channel Width (OC): 100μm or 200μm
    • Travel Width (T): 50 μm or 100μm
    • Pillar Spacing (Gap) (SP): 3μm or 8μm  
    • Pore sizes: 3μm or 8μm

     

    Design 2 (co-culture-Radial design with 3D tissue chamber)

    This configuration has a radial design as well and the pattern consists in a central chamber flanked by two outer channels. The central chamber has pillars of 25 µm diameter and spaced of 50 µm to create a scaffold suitable for the creation of a tissues with a 3D complex structure. The depth of all channels is 100 µm and a barrier region represents the intersection between them and the tissue chamber. The chamber and channels have inlets and outlets directly compatible with 1/16" Tygon tubing.

     

    Option A - Slit Barrier (suitable for liver-toxicology modeling)

    These devices have slits and gaps to form the barrier region between the outer channel and inner chamber.

    Here are the parameters of the available pattern:

    • Outer Channel Width (OC): 100 μm
    • Travel Width (T): 100 μm
    • Slit Spacing (SS): 50 μm
    • Slit Width: 2 μm

     

    Option B - Pillar Barrier (suitable for drug screening and inflammation modeling)  

    These devices have pillars forming the barrier region between the outer channel and inner chamber.

    Here are the parameters of the available patterns:

    • Outer Channel Width (OC): 200μm
    • Travel Width (T): 100μm
    • Pillar Spacing (Gap) (SP): 3μm or 8μm  
    • Pore sizes: 3μm or 8μm

     

    This setup includes:

    • 1x Microfluidic Chip

     

    Optional:

    • 1x Pack of 25 Clamps for the Tygon Tubing 1/16" OD. Clamps allow you to block the tubing of the unused inlets/outlets, this is for avoiding leakage from the tubing when you fill the chip or perform experiments in flow conditions

    • 1x Pack of 25 Needles Gauge (0.5" long) to connect the syringe of the pump directly with the tubing

    We offer different Microfluidics Chips, choose the design accordingly to your cell culture and create your own 3D tissue (see the Specifications).

    The complete setup comes sterile and have to be stored in dry conditions, without direct exposition to the sunlight at room temperature (15-25 °C). 

     

    Many co-culture protocols have been developed to establish true vascular monolayers in communication with tissue cells. Human cells grown in these chips retain a biological phenotype similar to that found in the real tissues. Leading researchers have validated that cells grown in these chips more accurately reflect the cell behavior found in vivo compared to cells grown using conventional culture techniques. 

    The available microfluidic platforms can be used to study cell/particle adhesion and cell-cell or cell-drug interactions and has been extensively validated across neuroscience, oncology, inflammation and toxicology applications.

    Unlike well-plate tests performed under static conditions, these chips reproduce the realistic dynamic conditions for the assessment of cell-drug and cell-cell interactions thereby providing an accurate in vitro platform to study and elucidate the mechanisms of success and failure. Compared to in vivo animal studies, they allow real-time visualization and analysis of the assay in a controlled environment.

    Under physiological fluid flow conditions it would be possible to study the interactions during:

    • Inflammation (leukocyte-endothelium),
    • Cancer development (tumor-endothelium),
    • Thrombosis (platelet-endothelium),
    • Infection (microbial-endothelium)

    3D Cancer models

    You can create a microfluidic 3D cell-based assay platform for quantitative assessments in a physiologically realistic tumor microenvironment. The system enables real time visualization and analysis of cell-cell and cell-drug interactions encompassing (a) transport across the vessel walls, and (b) delivery to the tumors. There are many areas of oncology research that can benefit by using these models. These include (1) basic research for understanding of the tumor microenvironment (cell viability, proliferation, invasion and tumor-stromal and tumor-endothelium interactions); and (2) drug screening for efficacy, toxicity and penetration.

    Chips with Design 1 allow to study cancer metastasis. This is a multi-step process that starts with the cancer cells leaving the original tumor site and migrating to distant parts of the body via the bloodstream or the lymphatic system. This process involves complex steps, including breaking of the extracellular matrix by the metastatic tumor cells, escape into the circulatory system, adhesion to the vascular wall at remote locations, followed by migration/invasion into tissue and subsequent proliferation.

    Chips with Design 2 are suitable for drug screening. Drugs or delivery systems (nanoparticles, polymers, liposomes, etc.) can be injected via the vascular channel or directly in the tumor chamber under both static and physiological fluid flow conditions and their responses can be observed in real-time mimicking the in vivo conditions. 

    3D tumor manual

    3D cancer model technical manual 

     

    Blood-Brain-Barrier model

    The BBB 3D model recreates the in vivo microenvironment by mimicking the histology of brain tissue cells in communication with endothelial cells across the BBB. Shear-induced endothelial cell tight junctions, which cannot be achieved in the Transwell® model, are easily achieved in the this model using physiological fluid flow. Interactions between brain tissue cells and endothelial cells are readily visualized in this assay. Transwell models do not allow real-time visualization of these cellular interactions, which are critical for understanding of the BBB microenvironment. 

    The Chip with Design 1 - option A (slit barrier) is a highly versatile platform for investigation of:

    • Tight junction proteins: Determine the levels of tight junction proteins namely zonula occludens, claudins and occludins which regulate the BBB.
    • Transporter proteins: Analyze functionality of transporter proteins (e.g. Pgp) in normal and dysfunctional BBB.
    • Drug permeability: Evaluate real-time permeability of therapeutics and small molecules across the endothelium of the BBB.
    • Inflammation: Understand the underlying mechanisms of  inflammatory responses on the regulation of the BBB.
    • Cell migration: Visualize and quantify in real-time migration of immune cells across the BBB.
    • Omic changes: Perform genomic, proteomic and metabolic analysis on normal and dysfunctional BBB.
    • Neurotoxicity: Analyze toxicity effects of chemical, biological and physical agents on the cells of the BBB.
    • Neuro-oncology: Investigate effects of the tumor cells on the BBB.

    Find here the related scientific publication to know more about this BBB 3D model: A Novel Dynamic Neonatal Blood-Brain Barrier on a Chip. S. Deosarkar, B. Prabhakarpandian, B. Wang, J.B. Sheffield, B. Krynska, M. Kiani. PLOS ONE, 2015 

    BBB model manual

    3D BBB model technical manual 

     

    Toxicology model 

    Current in vitro models use 2D monolayers or 3D aggregates of cells under static conditions for studying drug toxicity. These models fail to reproduce in vivo physiological features such as morphological size, physiological blood flow and cellular (biological) make-up of the specific organs being investigated. Other microfluidic models employ a membrane-based top-bottom two-compartment architecture, inherently limiting key desired features such as real-time visualization and the ability to simultaneous analyze multi-cellular cultures. 

    The chip with Design 1 and 2 - option A (slit barrier) is the only commercially available 3D toxicology model with real-time optical monitoring and multi-compartment, multi-cellular architecture and low reagent requirements. Other benefits of this platform are:

    • Physiologically realistic morphological, fluidic and 3D cellular conditions
    • Universal platform with architecture specific of desired organ
    • Significant reduction in cost and time
    • Robust and easy to use protocols
    • Compatible with standard analytical instruments for both on chip and off chip assays including omic methodologies for systems biology and bioinformatic analysis

     

    Tox model manuel

    Toxicology model technical manual 

     

    Inflammation model (Rolling, Adhesion and Migration Assay)

    The model has been developed to study the entire inflammation pathway in a realistic and dynamic environment. By creating a cell co-culture and a lumen of endothelial cells, the platform mimics a physiologically realistic model that includes flow and shear. The chip enables real-time tracking of rolling, adhesion and migration processes.

    With the Design 1 or 2 - option B (pillar barrier) you can create the inflammation model that provides a realistic testing environment including:

    • Physiological shear stress within a microvascular environment
    • In vivo like vascular morphology with fully enclosed lumen
    • Co-culture capability for cell-cell interactions
    • Quantitative real-time rolling, adhesion, and migration data from a single experiment

     

    Inflammation model manual

    Inflammation model technical manual 

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