Cell confluency reader

Microfluidic accessories

Cell confluency reader



Category: Microfluidic Other Accessories

Stop second-guessing the confluency of your cells on a small fraction of your cell culture vessel!


Automate your sub-culturing decisions with this affordable and compact imaging platform. It provides rapid and robust measurements of the confluency of your cells in the entire surface culture area and predicts the timing of your passaging.


Controlling cell confluency will maximize success of your cell transfection, differentiation and sub-culturing protocols.





 

On Demand

Our specialized microfluidics team will gladly help you define your needs
Stop second-guessing the confluency of your cells on a small fraction of your cell culture vessel!
Automate your sub-culturing decisions with this affordable and compact imaging platform. It provides rapid and robust measurements of the confluency of your cells in the entire surface culture area and predicts the timing of your passaging.
Controlling cell confluency will maximize success of your cell transfection, differentiation and sub-culturing protocols.
 

Automate data-driven decisions for sub-culturing: control and monitor reproducibly your cell confluency!

  • Real-time quantitative cell confluency

  • Confluency of the entire surface culture area

  • Predicts next passaging time

  • Records data for rigorous book keeping

         OPERATING PRINCIPLE

                             

                            Schematic drawing of the Cell Confluency Reader

    This affordable product  includes both software and imaging hardware and can therefore be purchased independently of your pre-existing microscopic equipment. This product will quantify, standardize, digitize and  monitor a crucial step in cell culture that is to this day often performed only qualitatively.

            

                          Representative work flow using the Cell Confluency Reader

    The user places the culture vessel of choice on the confluence reader that is equipped with cameras which capture high resolution images of the bulk of your culture surface. Within seconds, our algorithm segments the images; it quantifies the cell confluency and predicts its evolution so as to identify the timing at which your cell culture vessel will have reached your target confluency. The software notifies the user via email shortly before reaching the target confluency.

    PROBLEMS THIS SOLVES:

    •      Provides a quantitative measure of confluence

    •      Measures the real confluency of your entire cell population

    •      Enables control of confluency levels to avoid variability in results of downstream applications

    •     Identifies and notifies the passaging time to plan experiments ahead of time

    •      Digitizes and standardizes cell culture protocols

    RELEVANCE:

    • Passaging cells at variable and uncontrolled levels of confluence inevitably induces variability in your results. Confluency levels are known to be crucial for the health of cell, their differentiation and the success of their transfection. This product will help you precisely identify and predict the timing of your optimal cell confluency to standardize and increase control over the outcomes of your experiments.
    • Observing a couple of regions of your entire cell culture vessel is far from representative of the real confluency of your entire cell population. Our software analyzes within seconds the real confluency of most of your cell population. 
    • It stores your data to keep rigorous records of the proliferation and health of your cells across experiments. 

    IMPACT: 

    The routine use of this product will help you control reproducibly your cell confluency, which will have many impacts (listed in the figure below), amongst which:

    • maximize success of your downstream applications, such as cell transfection

    • improve the differentiation of your cells (stem cells, immune cells)

     

                

                                          Impact of Cell Confluency Reader on cell culture

     

    DETAILS:

    Use our equipment to take images of your culture vessel at multiple time points so that the proliferation rate your cells is estimated – our software (see screenshot below) uses real-time data to predict the timing of your ideal target confluency and the required split ratio according to your cell type. Our software draws information from a database that can guide you choosing a splitting ratio for sub-culturing depending on your cell type. You can store data to keep track of your cell stock proliferation rate and compare between flasks to identify more quickly anomalies within your stock. 

               

                                                 Representative screenshot of software

    This products works with numerous cell types, amongst which:

    • endothelial cells,
    • fibroblasts,
    • stem cells(i.e. iPS)
    • immune cells (monocytes, macrophages, T cells, dendritic cells)

          

    Contact us to discuss this product in more details with our product developer and manager, Alexandra Boussommier-Calleja.

                         Keep your cells happy and healthy!

     

    • Up to a total of 50 Mpixels
    • Resolution of 10 µm.
    • Custom-written software for automated image segmentation
    • Records and stores data for longitudinal measurements
    • Label-free, non-invasive measurement
    • Does not require any other consumable
    • Compatible via USB to any computer or tablet
    • Compact design: fits anywhere in your cell culture lab!
    • Compatible with numerous culture vessels: T150, T75, T25, T12.5, well-plates, cell culture dish
    • Compatible with numerous cell types, such as HUVECs, fibroblasts, cancer cells, stem cells, immune cells.
    •  Biology
    • Cell culture
    • Cell transfection
    • Stem cell differentiation
    • Pharmaceuticals
    • Drug discovery
    • Regenerative Medicine
    • In vitro assay development  

    Microfluidics contact team

    Contact our team:

    contact@darwin-microfluidics.com


    Our specialized team is at your service to help you pick the product best suited to your needs. We also offer specific quotes for high quantity, specific, or custom orders. Feel free to ask, that's what we're for!