Microfiltration and Cell Isolation Chip - Complete Setup
This modular microfluidic chip represents an innovative approach to create your own microfluidic platform for filtration and cell isolation. These chips were designed for reversible integration of various types of microfilters. The inlets and outlets with integrated Luer connections ensure leak-free junctions with the tubing and allow experiments under dynamic flow conditions.
The setup includes a Microfluidic Chip, a Clamp and a Microfilter! All you need to create your platform of microfiltration and cell isolation.
Three components characterize this microfluidic platform:
- Microfluidic chip
The modular microfluidic chip is designed for reversible integration of various types of filters and consists in two plastic plates in PC (Polycarbonate), each having four integrated magnets for reversible assembly and an elastomeric PDMS thin film to ensure good sealing up to pressures of 8 kPa. The Luer Connections in the upper part of the chip allow liquid perfusion and dynamic flow conditions during your experiments. The assembly is easy to use and with a reversible access to filter and isolated cells on chip for downstream analysis.
- Reversible patch integration thanks to the modular chip
- Leak-free Luer Connection molded with the chip
- Highly-resistant and optically clear material
- Standard microscope glass slide size
The microfluidic chip is available in three configurations:
a) Filter integration with tow connectors chip for trans- patch application, such as dead-end filtration or trans patch perfusion.
b) Filter integration with three connectors chip for trans- or cis- patch application, such as dead-end filtration, cross-flow filtration, etc.
c) Filter integration with four connectors chip for trans- or cis- patch application, such as dead-end filtration, cross-flow filtration, or air/liquid-liquid interface cell culture, etc.
When a filter is placed between the two plates, the force between the four pairs of magnets ensures auto-assembling at pressures up to 8 kPa.
The clamp, included in the pack, ensures good sealing with high flow pressures (up to 100 kPa). The chip can be placed in a hand screwed clamp for assembling and fast operation. At the end of your experiment in dynamic flow conditions, the filter can be easily removed by dissembling the clamp and the microfluidic chip.
Micro-engineered filters are available for high precision micro-particle or cell isolation. Unlike conventional filters, these filters are made of regular and high precision hole arrays realized by mass production. The shape, size and density of the holes are designed according to your specific needs. They can be used for the isolation of microorganisms, circulating tumor cells, fetal cells and endothelial progenitors in peripheral bloods.
The microholes can have different shape:
- Funnel shape
- 6 μm
- 5 μm
- 1 μm (only for funnel shape)
|Upper plate size||55 mm x 25 mm x 8 mm|
|Lower plate size||55 mm x 25 mm x 3 mm|
|Dimension for filter/patch chamber||Φ 13 X 0.2 mm²|
|Channel width||1000 μm|
|Chamber depth||500 μm|
|Material||PC with PDMS thin layer|
|Connection||Luer connectors (2, 3 or 4)|
|Dimension||80 mm x 25 mm x 3 mm|
|Number of windows||1|
|Window size||40 mm x 20 mm x 3 mm|
|Dimension||Φ 13 X 0.2 mm²|
The Complete Setup includes:
- 1x Microfluidic Chip with Luer Connectors
- 1x Clamp
- 1x Microfilter
The setup comes sterile and have to be stored in dry conditions, without direct exposition to the sunlight at room temperature (15-25 °C).
These chips are typically used for the integration a filter. Micro-chambers and micro-channels are predefined in both plastic plates to create a perfusable system when the setup is assembled.
The principle of microfiltration can be used to isolate single cells from complex suspensions by using a continuous fluid flow through the microfluidic chip.
Significative examples of filtration:
- giant cell trapping
- particle separation
- animal or bacterial cell isolation
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