NPG72 Polymer Starter Pack: alternative to PDMS

Microfabrication

NPG72 Polymer Starter Pack: alternative to PDMS



Category: Cell Culture Chips, Electrophoresis & Electrochemistry Chips, Microfabrication, Microfluidic Droplet Generators, Microfluidic Flow Cells, Microfluidic Organ-on-a-chip, Micromixers Chips

Try our brand new biocompatible polymer with this ready-to-fabricate start pack! The NPG72 is a great alternative to PDMS: self-sealing, resistant, cost-effective and biocompatible, it will greatly ease your work when fabricating chips. Contains 10 sheets of 15 x 15 cm NPG72 polymer.

Availability: In Stock
€330.00
Try our brand new biocompatible polymer with this ready-to-fabricate start pack! The NPG72 is a great alternative to PDMS: self-sealing, resistant, cost-effective and biocompatible, it will greatly ease your work when fabricating chips. Contains 10 sheets of 15 x 15 cm NPG72 polymer.

In this kit, you will be able to try the NPG72 with 10 sheets of 15 x 15 cm as an alternative to PDMS for your chip microfabrication. Different thicknesses are available upon request: 1.3 mm, 0.8 mm and 0.3 mm.

 

The NPG72 polymer is a real breakthrough for microfluidics

Features & Benefits

The NPG72 is a new polymer which gathers the advantages of PDMS without its drawbacks:

  • Biocompatible (ISO 10993-5 & UPS Class VI)
  • Self-sealing at ambient temperature: no need to use plasma bonding or high temperatures, you can then add biomaterials prior assembly
  • Cost-effective
  • Compatible for optical assays: low fluorescence, optically clear
  • No absorption, permeable to gas and not to liquid
  • Resistant to tearing: Unlike PDMS, it will not tear apart when you manipulate it

Effective integration, cellular and bio-reagents 

The high sealing performances of the NPG72 enable the integration of cells, proteins and others enzymes biomaterials prior to assembly. With a fabrication process that doesn’t involve plasma, vacuum or high-temperature, we can ensure that biological agents remain viable and functional in your device.

Fast microfabrication

  • Molding with a hot-embossing system
  • Low pressure: below 1 bar
  • No vacuum needed
  • Easy unmolding
  • Full operation takes less than a minute
  • Extremely low viscosity for rapid thermal molding with filling rates up to 100-1000 times greater than classical rigid thermoplastics (COC, PS, PMMA,…)

Applications

NPG72 is an alternative to any other chip material in the classical applications of microfluidic chips.

This polymer is particularly interesting in life sciences: its biocompatibility make it simple to design any experiment. The self-sealing technology is not destructive, unlike plasma, vacuum or high temperature used in the sealing of other chips.

Principal characteristics

NPG72 sheet dimensions 150 x 150 mm
Thickness 300 µm / 750 µm / 1.3 mm
Tear Strength 15 kN/m
Tensile Strength 7.6 kN/m
Elongation 720 %
Molding Temperature 115-185 °C

Self-sealing and fast bonding

The NPG72 is easy to bond to either NPG72 or glass after molding.

 

Bonding temperature NPG72 chip on NPG72 NPG72 chip on glass
Room temperature 600 mbar 400 mbar
85 °C 5.5 bar 720 mbar

 

 Contact us for more informations.

NPG72 sheet (x10): Available thicknesses of 1.3 mm, 0.8 mm and 0.3 mm. Dimensions of 15 x 15 cm.

  • Do I need a heat press for NPG72

Yes, most heat presses or hot-embossers should work. A press that can mold at a pressure of at least 7 psi or above is ideal (a vacuum assisted press is not required). You may have to make small adjustments to optimize molding your chips. We offer a compact microfabrication kit, user friendly and plug and play system. Contact us for more informations.

 

  •  What size range is reliably micromolded with NPG72?
The minimal microchannel width is 50 nm, and the depth range is 50 nm - 1 mm. However, microchannels between 1 µm and 1 mm are recommended with an aspect ratio (height to width) of 3:1 or less. Submicron structures and higher aspect ratios have also been achieved by some customers.
  • Which mold does NPG72 work best with?
Common molds used in the field of microfluidics work well. These include fragile molds, such as photoresists (e.g. SU‒8), and etched glass and silicon molds. Temperature resistant epoxy molds and traditional metallic molds (aluminum, nickel, brass molds) work also. Silicones such as PDMS work as well, however, be sure to choose a stiff silicone grade at least 20 Shore A durometers above NPG72 . We offer EPMO epoxy material to fabricate robust mold suited (up to 6’’ size) for NPG72 molding without the needs to treat it with anti-sticking agent.
  • How do I clean NPG72?
Isopropyl alcohol, methanol or distilled water may be used. NPG72 has to be dry (air or oven dried) before molding to avoid bubbling or splays (cloudiness). Tape is also recommended to remove dust particles. We recommend working with NPG72 in a clean room or under a laminar hood to minimize particle contamination.
  • Is the NPG72 appropriate for my fluorescence applications?
NPG72 material is a transparent material and has a transmittance of 50% at 295 nm and upon 90% in the visible region.
  • How do I mold NPG72 as transparent as possible ?
We recommend molding NPG72 using a very smooth mold. Metallic molds should have a mirror finish. Using glass or other transparent molds, such as epoxy or other silicones will ensure a more transparent result.
  • Why do I keep getting tiny bubbles in the plastic when I mold NPG72
Your setup is likely too hot or NPG72 is not completely dry. Lower the temperature, decrease molding time, air/oven dry NPG72, or perform molding in a low humidity condition to minimize bubbling. It may take some trial to optimize micromolding with NPG72 on your own setup.
  • What is the most effective way to sterilize NPG72?
Ethylene oxide, gamma irradiation or autoclave will work.
  • Is NPG72 microfluidic chips used for cell culture?
While NPG72 is less permeable than PDMS, it still has sufficient air permeability to sustain cell cultures. NPG72 grades have successfully been used for neurons, hepatocytes, endothelial, dermal, stem-cells and IPSCs cells cultures among others. NPG72 is UPS Class VI and ISO 10993-5 biocompatible certified material (PVC, Latex and others phthalates free).
  • How do I seal NPG72 microchannels to a substrate?
NPG72 is a self-sealing material that you can bond with usual microfluidics thermoplastics and materials such as PC, POC, PS, PP or glass, Si. The bonding process does not require plasma assisted or UV adhesive processes. It can be bonded at room temperature. To reduce the time of bonding and/or to improve the bonding strength, a thermal bonding process can be used (maximum 90°C). Customers have successfully sealed NPG72 by binding it to a substrate and storing the chip in an oven for minutes to overnight.
  • How do I control the hydrophilicity of NPG72 surfaces?
In its pristine state, NPG72 is slightly hydrophobic. To achieve stable hydrophilic surfaces strategies that have been used on hard plastics can also be applied to NPG72 surfaces. These strategies include plasma treatment or coatings with hydrophilic materials, such as poly (vinyl alcohol), polyurethane, polyacrylate, (hydroxypropyl) methyl cellulose, polyvinylpyrrolidone, or hyaluronic acid. Multiple vendors offer hydrophilic coatings through licensing models or outsourcing. Key points to consider are the cost structure, the type of coating, the coating application (e.g. dip, spray, spin, film coats), any curing step (e.g. UV or heat), and any post-processing steps (e.g. sterilization, cleaning, or storage conditions) that may affect your final chip.
  • How do I create inlet and outlet holes for my microfluidic channels?
Traditional biopsy punches used with silicones will not work here. The use of a rotary/hand punch or a laser cutter is recommended. Self-sealing connection pads have been developed and ensure easy and leak-free connections with any tubing.
  • How do I prevent nonspecific binding?
Methods traditionally used to treat hard plastics generally work with NPG72 as well. For example, coating the surfaces with animal caseins or BSA (bovine serum albumin) has been used with success.

Microfluidics contact team

Contact our team:

contact@darwin-microfluidics.com


Our specialized team is at your service to help you pick the product best suited to your needs. We also offer specific quotes for high quantity, specific, or custom orders. Feel free to ask, that's what we're for!