Organ-on-a-Chip - Complete Setup
- Product SKU: MS-Kit-O1 / PEGDA-Gelatin
- Category: Cell Culture Chips, MesoBioTech, Microfluidic Chips, Microfluidic Organ-on-a-chip, Standard microfluidic chips
This modular Organ-on-a-Chip platform represents an innovative approach to create 3D cell cultures and tissues with a precise and continuous control of culturing parameters. It is possible to mimic tissue organization, cell-cell interactions, extracellular matrix influence and physiological responses in a more in vivo-like environment. The inlets and outlets with integrated Luer connections ensure leak-free junctions with the tubing and allow experiments under dynamic flow conditions.
The setup includes Microfluidic Chips, a Clamp and Culture Patches! All you need to create your 3D cell culture.
An ideal Organ-on-a-Chip should be easy to use and with a reversible access to cells in chip for downstream analysis. Hence our Organ-on-Chip meets all these demands. Available in Small pack (1 chip - 1 clamp - 4 patches) or Large pack (4 chips - 1 clamp - 16 patches).
Three components characterize this microfluidic platform:
- Microfluidic chip
- Culture patch
The modular microfluidic chip consists in two plastic plates in PC (Polycarbonate), each of these has four integrated magnets for reversible assembly and an elastomeric PDMS thin film to ensure good sealing up to pressures of 8 kPa. The Luer Connectors in the upper part of the chip allow medium perfusion and dynamic flow conditions during your experiments of drug screening and/or cell differentiation.
The clamp, included in the pack, ensures good sealing with high flow pressures (up to 100 kPa). The chip can be placed in a hand screwed clamp for assembling and fast operation and, at the end of your experiment in dynamic flow conditions, the tissue patch can be easily removed by disassembling the clamps and the microfluidic chip.
The culture patch is a monolayer of nanofibers and support the organization of cells in a functional 3D microtissue. Culture patches are included in the setup and nanofibers can be made of two different natural polymers to mimic the extracellular matrix:
- collagen (mainly type I)
- PLGA poly(lactic-co-glycolic acid)
- PCL polycaprolactone
- PMGI polydimethylglutarimide
Thus, different patches can be used to culture cells or to differentiate Human Induced Pluripotent Stem Cells (hiPSC) in dish or microplates in order to create your tissue patch (e.g. cardiac, neuron, etc). Then, the obtained tissue patch (or other tissue culture inserts) can be integrated in the assembled Organ-on-Chip. A co-culture of two different cell types is possible as well. In order to seed the cells on both sides a coating of matrigel and collagen is needed. The ratio of the mix will be cell type dependent.
Once the assembly is completed, micro-chambers and micro-channels are patterned in both plastic plates of the chip to create two independent microfluidic systems: the upper and the lower chambers separated by the tissue patch. These two microfluidic chambers can be perfused with different culture media. Thus a tissue interface can be formed to simulate alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc. Tissues inside the chip can be observed by microscopy or harvested for downstream analysis.
|Upper plate size||55 mm x 25 mm x 8 mm|
|Lower plate size||55 mm x 25 mm x 3 mm|
|Dimension for patch insert||Φ 13 X 0.2 mm²|
|Channel width||1000 μm|
|Chamber depth||500 μm|
|Material||PC with PDMS thin layer|
|Materials||Natural or Synthetic biocompatible polymers|
|Dimensions||Φ 13 X 0.2 mm²|
|Nanofiber diameter||100-500 nm|
|Nanofiber thickness||< 1 µm|
|Dimension||80 mm x 25 mm x 3 mm|
|Number of windows||1|
|Window size||40 mm x 20 mm x 3 mm|
The Small Pack setup includes:
- 1x Microfluidic Chip with four Luer Connectors (Upper plate + Lower plate)
- 1x Clamp
- 4x Culture Patches
The Large Pack setup includes:
- 4x Microfluidic Chips with four Luer Connectors (Upper plate + Lower plate)
- 1x Clamp
- 16x Culture Patches
We offer different Culture Patches, choose it accordingly to your cell culture and create your own 3D tissue.
The complete setup comes sterile and have to be stored in dry conditions, without direct exposition to the sunlight at room temperature (15-25 °C).
Integration of a tissue patch into the chip gives rise to Organ-on-Chip models. Typically, a tissue patch made of two types of cells can be inserted in the interface of two elastoplastic plates. After clamping, solutions can injected to create physiological conditions or test the effects of drugs and growth factors.
- Reversible patch integration thanks to the modular chip
- Leak-free Luer Connection molded with the chip
- Highly-resistant and optically clear material
- Standard microscope glass slide size
By changing the cell type and the patch, this chip can be used to create:
- Neural or cardio-vascular networks-on-a-chip,
- Mesenchymal stem cells (MSC) or bone marrow-on-a-chip,
- ESC or iPSC-derived stem cells (ESC/iPSC)-on-a-chip.
This modular microfluidic platform significantly improve the predictability of drug screening and studies of personalized medicine.
Useful cell culture protocols can be find in these papers published in high impact factor journals:
- Tang et al., Effective motor neuron differentiation of hiPSCs on a patch made of crosslinked monolayer gelatin nanofibers. J. Mater. Chem. B, 2016, 4, 3305 (PDF file)
Figure 1. Neuronal cells in red ( Neuron-specific class III beta-tubulin TUJ1 positive) and Astrocytes in green (glial fibrillary acidic protein GFAP positive)
- Tang et al., Induction and differentiation of human induced pluripotent stem cells into functional cardiomyocytes on a compartmented monolayer of gelatin nanofibers. Nanoscale, 2016, 8, 14530 (PDF file)
- Performing an experiment under dynamic flow conditions is possible in many different ways, it depends on the type of results you aim to obtain:
- If your experiment requires a short exposure time you can perform it without incubator by just keeping the medium at the right temperature and using CO2 independent medium (Leibovitz L-15 for example);
- If your experiment requires a prolonged incubation you can put the chip in the incubator and leave the perfusion system outside; the use of thin tubes (1/16" OD as an example) can facilitate the task because they can pass through the incubator's door without perturbing the correct closure.
- The sterilization of the components is possible by using a solution of 70% ethanol or by UV exposition (under the UV light of the hood for example).
- It would be possible to reuse the chip. However, we recommend to not use the chip with different cells in order to avoid any possible cross contamination. In our test, we use the same chip often with different patches of the same cells in culture but with some limitations: the chip has some PDMS parts inside and the prolonged use could increase the absorption of chemicals with possible toxic effects for the cells. The chip should be reused 3-4 times but this will strongly depend on your specific working conditions.
- You can remove bubbles from the device by priming it with culture medium. It is possible to inject the medium in the empty device with increasing pressures until bubbles are flushed away. We recommend to perform this process before to seed the cells. This should be easy if you seed the cells on the support once the device is assembled.
On the other hand, if you seed the cells on the support and then put it in the device, you can try to inject the media once the device is assembled. We suggest to start with a very low pressure and then to increase slowly the pressure and verify that the cells are not detaching from the support.
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