Primovert iLED Compact Inverted Microscope
Rated 3.5/5 based on 11 customer reviews

Primovert iLED Compact Inverted Microscope

This compact and versatile inverted microscope from ZEISS has been specifically configured to meet your needs for your daily experiments both in microfluidics and cell culture, including fluorescence observation (470 nm LED). Coupled with a camera, it is a cost-effective yet powerful instrument for fast and efficient experiments.
Microscope with binocular phototube for brightfield, phase contrast and basic fluorescence (470 nm), including a set of 4 objectives (4x, 10x Ph1, 20x Ph1, 40x Ph1), condenser 0.4, object guide and universal mounting frame. All the accessories are included to work out-of-the-box.
Camera C-mount adapter available as an option for common sensors sizes (0.5x and 0.65x). Camera not included.
Average lead time: 6-8 weeks
€8,687.00
This compact and versatile inverted microscope from ZEISS has been specifically configured to meet your needs for your daily experiments both in microfluidics and cell culture, including fluorescence observation (470 nm LED). Coupled with a camera, it is a cost-effective yet powerful instrument for fast and efficient experiments.
Microscope with binocular phototube for brightfield, phase contrast and basic fluorescence (470 nm), including a set of 4 objectives (4x, 10x Ph1, 20x Ph1, 40x Ph1), condenser 0.4, object guide and universal mounting frame. All the accessories are included to work out-of-the-box.
Camera C-mount adapter available as an option for common sensors sizes (0.5x and 0.65x). Camera not included.

In partnership with ZEISS, Darwin Microfluidics has designed a Primovert setup for your everyday experiments in microfluidics.

This setup comprises the essential features expected for a routine inverted microscope used in the field of microfluidics. It has a small footprint, and yet provides a large space to set your experiment on the stage with a 0.4 condenser (55 mm of working distance, can be removed). The included 470 nm LED enables easy fluorescence observation, for ant label in the range of GFP, FITC, fluorescein, etc. Using the provided object guide, you will be able to find your area of interest and get it precisely in the field of view.

We have chosen a set of objectives with magnification from 4x to 40x, giving you the ability of seeing the common microfluidics objects, like droplets. For better contrast, objectives from 10x to 40x have Phase contrast, sharing a single phase ring (Ph1). It can also be used to check on your cells!  

This microscope comes with a binocular phototube, letting you fit a camera, for which we also provide adapters as an option.

You can find more details and applications in the brochure

 

Just what you need & easy-to-use

 

Primovert long working distance condenser Benefit from a large working area on the microscope stage thanks to the long working distance 0.4 condenser (55 mm), while keeping an excellent resolution in transmitted light. 
Primovert phase slider Using Phase Contrast is made easy on this Primovert configuration, as there is a single slider with PH1 for 10×, 20× and 40× magnification. Thus, there is no need to adjust the phase position when you change the magnification.
Primovert LED Illumination LED illumination gives you the benefit of long life and stable colour temperature, without the need for any alignment. Using LED also prevents from heating the sample.
Primovert fluorescence switch Everything about Primovert is designed to facilitate
your daily work. Use the switch on the stand to
shift effortlessly from phase contrast to fluorescence
contrast.
Primovert mounting frames You can use various mounting frames and stage adjustment for flasks and multi-well plates. For many Petri dishes, you can also expand the stage.
Primovert Software (optional)
Use the free ZEN lite microscope software to control ZEISS microscope cameras, capture images or view your CZI files.


 

Applications

HeLa Cells Primovert  U2OS cells GFP fluorescence on Primovert

Left: HeLa cells. Magnification 20×, phase contrast.
Right: U2OS cells. Magnification 20x, GFP fluorescence contrast.

  • Use the nosepiece with multiple objectives to change the magnification 4 – 40×, phase ring.

Primovert has a 4-position nosepiece and we have selected Plan-Achromat (4x/0.10 and 10x/0.25) and LD Plan-Achromat (20x/0.3 and 40x/0.5) objectives adapted to your experiments, with the same phase ring for magnifications from 10x to 40x. 

All the selected objectives have long working distance 

  • Use the microscope over several hours.

In automatic mode, Primovert operates in standby. If the device is not used for 15 minutes, it automatically shuts itself off.
Simply press a button to reactivate it.

  • Evaluate unstained, transparent samples such as living cells or droplets.

Primovert is equipped with phase contrast. You use a universal phase slider with only two position: BF and PH1 for 10x, 20x, and 40x magnification. There is then no need for adjusting the phase ring position when changing the magnification.

  • Excite and observe GFP, FITC or fluorescein.

With Primovert iLED, you can switch between brightfield and fluorescence contrast directly on the stand and evaluate both unstained and GFP-labeled cells.
The LED fluorescence provides even illumination of the sample. You avoid long warming up and cooling down phases as well as lamp adjustments.

  • Use the microscope in a sterile or dust-free environment.

Primovert’s compact design enables the microscope to fit into any cell culture laboratory. You can put Primovert straight in your laminar flow hood.

  • Use various sample holders.

Primovert comes with an object guide and a universal mounting fame, enabling you to fit different kind of sample holders on the stage. You can use standard microscopy slides, standard format microfluidics chips, but also custom size chips or Petri dishes.

 

Primovert dimensions

 

Dimensions (width x depth x height)

Approx. 215.5 mm × 552 mm × 494 mm

Weight (without accessories or packaging)

Approx. 11.5 kg

 

Ambient conditions

 

Transportation (in packaging)

Permissible ambient temperature

−40°C to +70°C

Storage

Permissible ambient temperature

+10°C to +40°C

Permissible humidity

Max. 75% at 35°C (without condensation)

Operation

Area of use

Closed spaces

 Max. altitude

2,000 m

Permissible ambient temperature

+10°C to +40°C

Permissible humidity

Max. 75 % at 35°C (without condensation)

 

Technical specifications

 

Protection class

II

Protection type

IP20

Electrical safety

Pursuant to DIN EN 61010-1 (IEC 61010-1) and in accordance with CSA and UL standards

Degree of pollution

2

Overvoltage category

II

Radio interference suppression

Pursuant to EN 61326-1, EN 61326-2-101

 Main voltage

100 to 240 V (±10%); thanks to the worldwide power adapter, adjusting the voltage of the device is not required

Power frequency

50/60 Hz

Microscope 12 V / 6 V DC

Adjustable 1.5 V to 6 V

LED class of entire device

Risk group 2 pursuant to IEC 62471

 

Light source

 

LED illumination

White-light LED, peak wavelength 450 nm, LED risk group 2 pursuant to IEC 62471

Fluorescent illumination

Blue LED, peak wavelength 470 nm, LED risk group 2 pursuant to IEC 62471

Homogeneous image field illumination

20 mm diameter

Analog brightness adjustment from

Approx. 15 to 100 %

Constant color temperature independent of brightness

7,000 K

 Homogeneous image field illumination

20 mm diameter

Analog brightness adjustment from

Approx. 15 to 100 %

With field of view of 20

WF 10×/20 Br. foc.

 

Optical and mechanical data

 

Maximum field of view

20

Illumination

Epi-fluorescence / transmitted light

Fluorescence source

LED wavelength 470 nm

Transmitted light source

LED 7,000 K

Eyepiece distance (pupil distance)

Adjustable from 48 to 75 mm

Viewing angle

45°

Viewing height

350 to 390 mm

Visual output

Tube factor 1x

Stand with stage focus

Using coarse adjustment

45 mm / rev

Using fine adjustment

0.5 mm / rev

Total lift

15 mm

Switching objectives

Manually using 4x nosepiece turret

Objectives

First-class infinity-focus objective range with screw thread W 0.8

Eyepieces with field of view of 20

30 mm plug-in diameter, WF 10× / 20 Br. foc.

Object stage

Permanently installed

Dimensions (width × depth)

200 mm × 239 mm

Stage adjustment

Right

Verniers with number and letter scale

X-axis: number scale; read from right to left; y-axis: letter scale, read using the mirror

Coaxial drive

Right

LD condenser 0.4

for Vobj 4× to 40×, a = 55 mm

With field of view of 20

WF 10×/20 Br. foc.

ZEISS Primovert iLED microscope stand with binocular phototube

Primovert iLED
  • 4-position objective nosepiece with a selection of 4 objectives (see specs below)
  • Binocular phototube 45°/20 (50%/50%) with eyepieces 10x/20
  • Transmitted-light illumination with white-light LED
  • Long working distance condenser (0.4 / 55 mm) with 2-position Ph slider (empty, Ph1)
  • Epi-fluorescence illumination with 470 nm LED and GFP-like fluorochromes observation
  • Specimen stage 200x239 mm with object-guide 108x72mm, right-operated, coming with universal mounting frame for precise positioning of slides and dishes
  • External power unit 100...240VAC/50...60Hz with country-specific adapters
  • Dust cover
  • Camera C-mount adapter optional (0.5x or 0.65x)

Objectives specifications (Plan-Achromat class, for Primovert)

 Objective Numerical Aperture Working distance Phase contrast
4x 0.10 12.0 mm -
10x 0.25 4.4 mm Ph1
20x 0.30 4.6 mm Ph1
40x 0.50 2.7 mm Ph1


While buying your microscope at Darwin Microfluidics, benefit of the amazing ZEISS customer service

 

ZEISS Customer Service

 

Because the ZEISS microscope system is one of your most important tools, we make sure it is always ready to perform. What’s more, we’ll see to it that you are employing all the options that get the best from your microscope. You can choose from a range of service products, each delivered by highly qualified ZEISS specialists who will support you long beyond the purchase of your system. Our aim is to enable you to experience those special moments that inspire your work.

 

Repair. Maintain. Optimize.

Attain maximum uptime with your microscope. A ZEISS Protect Service Agreement lets you budget for operating costs, all the while reducing costly downtime and achieving the best results through the improved performance of your system. Choose from service agreements designed to give you a range of options and control levels. We’ll work with you to select the service program that addresses your system needs and usage requirements, in line with your organization’s standard practices. Our service on-demand also brings you distinct advantages. ZEISS service staff will analyze issues at hand and resolve them whether using remote maintenance software or working on site.

 

Enhance Your Microscope System.

Your ZEISS microscope system is designed for a variety of updates: open interfaces allow you to maintain a high technological level at all times. As a result you’ll work more efficiently now, while extending the productive lifetime of your microscope as new update possibilities come on stream.

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Payment Terms

You can either order and pay via credit card or invoice (through a purchase order). Request a quote easily by clicking on the "Request a Quote" button in the cart page.

Please note that some products are not available for direct purchase: just click "Request a Quote" on the product page and fill in the form to get more informations!

Credit card 

  • Add your products to the cart
  • In the cart page, click on Proceed to Checkout
  • Complete your credit card informations (don't worry, we are using Stripe, a PCI-certified program specialized in online transactions)
  • That's it! You will receive an order confirmation within a few minutes, and our team will send you the invoice.

Invoice

  • Add your products to the cart
  • In the website header, click on Request a Quote
  • Fill in the required informations, then click on Submit Quote
  • Our team will send you a quote shortly
  • After receiving the quote, just send your purchase order at contact@darwin-microfluidics.com
  • Our team will complete your purchase and send you the invoice!

 

If you wish to pay in USD, GBP or AUD, please specify it in your PO. We can add the converted value in the required currency to the quote if requested.

 

Worldwide shipping

We ship worldwide from our warehouse in France.

Shipping charges are calculated based on the shipping country, and can be estimated from the cart page. Our shipping rates and times are:

  • France: GLS (1-2 business days), free shipping above 350€
  • Germany, Austria, Belgium, Denmark, Spain, Hungary, Italy, Luxembourg, Netherlands, Poland, Portugal, Czechia, Slovakia:
    • GLS (2-4 business days), free shipping above 350€
    • DHL Express (1-2 business days), reduced rate above 350€
  • Bulgaria, Croatia, Estonia, Finland, Ireland, Latvia, Lithuania, Romania, Slovenia, Sweden, Greece, Cyprus, Malta: DHL Express (1-2 business days), free shipping above 400€
  • United Kingdom: DHL Express (1-2 business days), free shipping above 400€
  • USA, Canada: DHL Express (1-3 business days), free shipping above 400€
  • Switzerland: DHL Express (1-2 days), free shipping above 450€
  • Other countries: DHL Express, shipping rates based on the destination, weight and dimensions

 

If you wish to use your own shipping account, we will charge a flat fee of 15€.

We reserve the right to apply a surcharge for parcels > 30 kg, palletized shipments, or packages with dimensions exceeding the maximum dimensions of our carriers.

Please note that our standard shipping incoterms are DAP (Delivered At Place), or EXW if using your own carrier.

Contact us if you have any questions!

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