MEPS-TBC-WL organ-on-a-chip (pack of 12)
The tissue-to-tissue barrier chip MEPS-TBC-WL (lower well type), is a human tissue culture platform that allows the modeling of human physiology and pathology of key organs at a tissue level. The platform, manufactured by high-precision injection molding, comprises one microfluidic channel and one chamber divided by a porous membrane. The bottom well reservoir allows for the construction of a 3D tissue parenchymal environment. This pack of 12 chips provides 96 replicate units for high-throughput experiments.
General
This chip displays 8 identical and independent designs for 2D-3D hybrid tissue-to-tissue barrier modeling. The chip is manufactured in polystyrene and features a microfluidic channel on the upper layer and a large chamber on the bottom layer, separated by a polyester membrane with a 3 µm pore size. The vascular endothelium is constructed on the upper side of the membrane with shear stress, and a 3D perivascular multicellular environment is formed beneath the membrane. This environment can include 3D matrices, hydrogel-mixed cell populations, organoids, and patient-derived tissues to model complex human tissue architecture.
The pump-free, ready-to-use platform allows high-precision sampling for drug testing and disease modeling. It can be operated with the automated microphysiological system ProMEPS, a game-changer robotic system for efficient and precise drug safety and effectiveness assessment. The platform is composed of an insert, a base, and a sampling plate. The inlets and outlets are also compatible with a 1 µL pipette tip.
It is recommended to use the chip clamp comprised in the starter kit for this platform to avoid any air and solution leakage.
A wide range of applications can be explored with the MEPS-TBC-WL, such as 3D blood-brain barrier, neurovascular, hepatic, skin models.
Content
12x MEPS-TBC-WL chips (individually wrapped and sterile)
Specifications
| Applications | Perfused 2D/3D tissue-tissue co-culture | |
| Materials | Polystyrene | |
| Number of units per chip | 8 | |
| Dimensions | 30 mm x 75 mm | |
| Base | Channel width x height | 1000 µm x 3000 µm |
| Well surface area | 4 mm x 1 mm | |
| ECM-gel well volume | 2~14.35 µL | |
| Insert | Channel width x height | 500 µm x 100 µm |
| Inlet/outlet diameter | ⌀ 2.5 mm | |
| Perfusion channel volume | 0.3 µL | |
| Membrane | Pore size/density | 3 µm / 6E+05 cm² |
| Materials | Polyester | |
| Thickness | 9 µm | |
| Co-culture interface surface | 1.14 mm² | |
| Sampling plate | Plate width x height | 900 µm x 2900 µm |
| Containing volume | 50 µL in each well | |
Documentation
Protocol will be provided via email after purchase.
Ahn, S. I., Sei, Y. J., Park, H. J., Kim, J., Ryu, Y., Choi, J. J., Sung, H-J., MacDonald, T. J., Levey, A. I. & Kim, Y. (2020). Microengineered human blood-brain barrier platform for understanding nanoparticle transport mechanisms. Nature Communications,11, 175. https://doi.org/10.1038/s41467-019-13896-7
Kim, J. I., Yoon, T., Kim, P., Bekhbat, M., Kang, S. M., Rho, H. S., Ahn, S. I. & Kim, Y. (2023). Manufactured tissue-to-tissue barrier chip for modeling the human blood-brain barrier and regulation of cellular trafficking. Lab on a Chip,23(13), 2990-3001. https://doi.org/10.1039/D3LC00124E
The tissue-to-tissue barrier chip MEPS-TBC-WL (lower well type), is a human tissue culture platform that allows the modeling of human physiology and pathology of key organs at a tissue level. The platform, manufactured by high-precision injection molding, comprises one microfluidic channel and one chamber divided by a porous membrane. The bottom well reservoir allows for the construction of a 3D tissue parenchymal environment. This pack of 12 chips provides 96 replicate units for high-throughput experiments.
General
This chip displays 8 identical and independent designs for 2D-3D hybrid tissue-to-tissue barrier modeling. The chip is manufactured in polystyrene and features a microfluidic channel on the upper layer and a large chamber on the bottom layer, separated by a polyester membrane with a 3 µm pore size. The vascular endothelium is constructed on the upper side of the membrane with shear stress, and a 3D perivascular multicellular environment is formed beneath the membrane. This environment can include 3D matrices, hydrogel-mixed cell populations, organoids, and patient-derived tissues to model complex human tissue architecture.
The pump-free, ready-to-use platform allows high-precision sampling for drug testing and disease modeling. It can be operated with the automated microphysiological system ProMEPS, a game-changer robotic system for efficient and precise drug safety and effectiveness assessment. The platform is composed of an insert, a base, and a sampling plate. The inlets and outlets are also compatible with a 1 µL pipette tip.
It is recommended to use the chip clamp comprised in the starter kit for this platform to avoid any air and solution leakage.
A wide range of applications can be explored with the MEPS-TBC-WL, such as 3D blood-brain barrier, neurovascular, hepatic, skin models.
Content
12x MEPS-TBC-WL chips (individually wrapped and sterile)
Specifications
| Applications | Perfused 2D/3D tissue-tissue co-culture | |
| Materials | Polystyrene | |
| Number of units per chip | 8 | |
| Dimensions | 30 mm x 75 mm | |
| Base | Channel width x height | 1000 µm x 3000 µm |
| Well surface area | 4 mm x 1 mm | |
| ECM-gel well volume | 2~14.35 µL | |
| Insert | Channel width x height | 500 µm x 100 µm |
| Inlet/outlet diameter | ⌀ 2.5 mm | |
| Perfusion channel volume | 0.3 µL | |
| Membrane | Pore size/density | 3 µm / 6E+05 cm² |
| Materials | Polyester | |
| Thickness | 9 µm | |
| Co-culture interface surface | 1.14 mm² | |
| Sampling plate | Plate width x height | 900 µm x 2900 µm |
| Containing volume | 50 µL in each well | |
Documentation
Protocol will be provided via email after purchase.
Ahn, S. I., Sei, Y. J., Park, H. J., Kim, J., Ryu, Y., Choi, J. J., Sung, H-J., MacDonald, T. J., Levey, A. I. & Kim, Y. (2020). Microengineered human blood-brain barrier platform for understanding nanoparticle transport mechanisms. Nature Communications,11, 175. https://doi.org/10.1038/s41467-019-13896-7
Kim, J. I., Yoon, T., Kim, P., Bekhbat, M., Kang, S. M., Rho, H. S., Ahn, S. I. & Kim, Y. (2023). Manufactured tissue-to-tissue barrier chip for modeling the human blood-brain barrier and regulation of cellular trafficking. Lab on a Chip,23(13), 2990-3001. https://doi.org/10.1039/D3LC00124E