The HUMIMIC Chip3 and Chip3plus are cutting-edge organ-on-a-chip platforms allowing the simultaneous culture of three tissue types in a single microfluidic circuit. It provides a more realistic and dynamic model of human physiology. This 3-organ platform enables the study of complex inter-organ interactions, drug responses, and disease mechanisms in a controlled, miniature environment.

General

The HUMIMIC Chip3 and Chip3plus allow the co-culture of up to 3 different tissues in a joint microfluidic circuit to develop Microphysiological Systems (MPS). The HUMIMIC Chips are operated via the HUMIMIC Starter, which can control several chips and chip types at the same time. Pressure and vacuum can be applied and allow the modeling of a lifelike supply of the tissues and also the modulation of shear stress according to the experimental needs of the user. The HUMIMIC Chip2 contains 3 pump membranes (500 µm thick) to control the liquid displacement in the microfluidic circuit, through valves activated by the pressure applied by the HUMIMIC Starter.

The flexibility of the HUMIMIC device allows to study any organ and also biological barrier models like the intestine, the lung or the skin. It is compatible with cell line, primary cells but also biopsies, tissue sections or spheroids. It is also possible to use commercially available culture inserts or even matrix-suported cultures to mimick the 3D environment of the organs. These chips, made of polycarbonate, PDMS and glass, have been designed for long-term experiments allowing the study of disease models and drug's efficacy and toxicity.

The Chip3 devices exists in 3 formats, displaying different sizes of culture compartment to adapt to the studied organs and tissue types:

• The Chip3 displaying two "96-well" (6.5 mm of diameter) and one bigger "24-well" (14 mm of diameter)
• The Chip3plus with one "96-well" and two "24-well"
• Here we propose two different versions of the Chip3plus, one for regular tissue studies and one to mimick air-liquid interfaces (Chip3plus-ALI).

Each chip is provided with the relevant culture compartments which are screwed into the device thanks to a hexagonal socket wrench. For media exchange, the compartments of one circuit need to be opened separately.

The types of culture compartment proposed for each chip are described in the specification section. Note that the nature of the culture compartment can be changed according to what is described in the product catalog of the Documentation section.

All chips are provided prefilled with DPBS supplemented with 1% Penicillin/Streptomycin (PS) but can alos be provided with a mix of 0.01% Gentamicin / 0.1% Amphotericin B (GA) or without any antibiotics (WO) added in the DPBS. For any requests regarding customization, please contact us. 

Content

1x HUMIMIC Chip3 or Chip3plus (including cell culture compartments)

Specifications

• Chip3:
  • 1x Threadfit 01: compatible with Transwell inserts or medium reservoir of up to 350 µL
  • 1x Threadfit 03: compatible with scaffolds or medium reservoir of up to 750µL 
  • 1x Threadfit 07: compatible with Transwell inserts 
• Chip3plus: 
  • 1x Threadfit 03: compatible with scaffolds or medium reservoir of up to 750µL 
  • 1x Threadfit 07: compatible with Transwell inserts  
  • 1x Threadfit 08: medium reservoir of up to 3 mL 
• Chip3plus - ALI: 
  • 1x Threadfit 03: compatible with scaffolds or medium reservoir of up to 750µL 
  • 1x Threadfit 07: compatible with Transwell inserts 
  • 1x Airfit 06: compatible with Transwell inserts, with airpermeable lid, recommended for air exposed tissue models. Can only be used once per circuit.

Technical drawing of the Chip3

Technical drawing of the Chip3plus

Documentation

? HUMIMIC Chip3 - Quick Guide

? HUMIMIC Chip3plus - Quick Guide