SynALI Air-Liquid Interface Lung Model
- Product SKU: SY-405001
- Category: Microfluidic Chips, Microfluidic Organ-on-a-chip, Organ-on-a-chip Bundles, SynVivo
This organ-on-a-chip with central chamber and flanked microchannels allows to create an air-liquid interface model mimicking the lung architecture. The microfluidic chip can be functionalized with lung epithelial cells surrounded by a vasculature made of endothelial cells to recreate the typical in vivo Air-Liquid Interface.
Chip only, or available in two kit sizes to start using the setup out of the box!
SynALI from SynVivo is a new air-liquid interface model (Air Liquid Interface Microfabricated Porous Structures Ciliated Airway Cells - ALI) imitating the architecture of the lungs. The microfluidic device is optically clear, functionalized with epithelial cells surrounded by a vascular system composed of endothelial cells. This structure maintains an air fluid (ALI) through the cells of the airways, allowing the formation of respiratory tubules that transport mucus: this is the phenomenon of mucociliary clearance.
Many parameters can be observed and quantified in real time with this totally innovative device, such as the morphology of the cells, the structure of the airways, the interactions between cells and the different functions of the airways (mucus transport, ciliary beating) and the therapeutic improvement induced.
- Realistic airway structure and environment
- In vivo hemodynamic shear stress
- Real-time visualization of cellular and barrier functionality including mucus, ciliary beating, immune cell interactions and therapeutic screening
- Robust and easy to use protocols
To run SynALI assays, two kits formats are available (kits do not include the air pump needed to establish the air-liquid interface):
|Starter Kit||Assay Kit|
To only order the chip, choose "Chip only" in the kit selection.
IMN2 linear chip:
The Airway model can be created with a co-culture of endothelial and epithelial cells and an Alveolar tri-culture model with endothelial, epithelial and fibroblasts is possible as well.
Air Liquid Interface model technical manual
These co-culture protocols have been developed to establish true vascular monolayers in communication with the 3D lung tissue. Human cells grown in these chips retain a biological phenotype similar to that found in the real tissue. Leading researchers have validated that cells grown in these chips more accurately reflect the cell behavior found in vivo compared to cells grown using conventional culture techniques.
Unlike well-plate tests performed under static conditions, these chips reproduce the realistic dynamic conditions for the assessment of cell-drug and cell-cell interactions thereby providing an accurate in vitro platform to study and elucidate the mechanisms of success and failure. Compared to in vivo animal studies, they allow real-time visualization and analysis of the assay in a controlled environment.
An example of a confluent endothelial cell seeding density in one of the outer channels
An example of the microfluidic device with lung epithelial cells immediately after seeding.
Mucus formation and biomarker staining: A) - C) Confluent co-culture of endothelial and epithelial cells; mucus formation and staining of biomarkers in epithelial cells. D) and E) Biomarker staining for tight junction markers (VE-Cadherin and ZO-1) in endothelial cells.
Liu Z. et al., Co-cultured microfluidic model of the airway optimized for microscopy and micro-optical coherence tomography imaging, Biomedical Optics Express 2019 (Download)
(a) The ALI device to develop the air-liquid interface across the cells. The
air (or epithelial) channel is separated from two fluid (basolateral) channels by pores. Right panel shows conceptual drawing of the proposed orientation of the cells when seen from top (above) and a cross-section (below). (b) Bright Field image of the fabricated device without cells and the magnified view showing the pores. (c) Fabricated device bonded to the glass coverslip with inserted tubing for perfusion.
(a-c). Phase contrast imaging
of HBE cells in the center channel: (a) Directly after seeding, (b) attachment of cells after 24 hours, and (c) 100% confluence after 7 days of culture. (d) Live/dead staining. (e) Cross-sectional view of 3-D reconstructed confocal image (10X mag.). Cell culture is
co-stained with Plasma Membrane Orange and Calcein Green. (f). En face of Fig. 3(e).
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