SynALI Air-Liquid Interface Lung Model
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SynALI Air-Liquid Interface Lung Model

This organ-on-a-chip with a central chamber and flanked microchannels allows to create an air-liquid interface model mimicking the lung architecture. The microfluidic chip can be functionalized with lung epithelial cells surrounded by a vasculature made of endothelial cells to recreate the typical in vivo Air-Liquid Interface.
Chip only, or available in two kit sizes to start using the setup out of the box!
Average lead time: 3 weeks
€315.00
This organ-on-a-chip with a central chamber and flanked microchannels allows to create an air-liquid interface model mimicking the lung architecture. The microfluidic chip can be functionalized with lung epithelial cells surrounded by a vasculature made of endothelial cells to recreate the typical in vivo Air-Liquid Interface.
Chip only, or available in two kit sizes to start using the setup out of the box!

SynALI from SynVivo is a new air-liquid interface model (Air Liquid Interface Microfabricated Porous Structures Ciliated Airway Cells - ALI) imitating the architecture of the lungs. The microfluidic device is optically clear, and functionalized with epithelial cells surrounded by a vascular system composed of endothelial cells. This structure maintains an air-fluid (ALI) through the cells of the airways, allowing the formation of respiratory tubules that transport mucus: this is the phenomenon of mucociliary clearance.

Many parameters can be observed and quantified in real-time with this totally innovative device, such as the morphology of the cells, the structure of the airways, the interactions between cells, and the different functions of the airways (mucus transport, ciliary beating), and the therapeutic improvement induced.

  • Realistic airway structure and environment
  • In vivo hemodynamic shear stress
  • Real-time visualization of cellular and barrier functionality including mucus, ciliary beating, immune cell interactions, and therapeutic screening
  • Robust and easy-to-use protocols

To run SynALI assays, two kits formats are available (kits do not include the air pump needed to establish the air-liquid interface):

Starter Kit Assay Kit
  • 10 SynALI chips -IMN2 linear (3 µm slits)
  • Accessories including tubing, clamps, needles and syringes
  • Pneumatic priming device (required for priming tubing to remove air)
  • 10 SynALI chips – IMN2 linear (3 µm slits)
  • Accessories including tubing, clamps, needles and syringes

To only order the chip, choose "Chip only" in the kit selection.

Examples of models developed using SynALI

Small airway lung model

Small airway lung model - Co-culture with human bronchial epithelial cells (HBEC) and lung microvascular endothelial cells.

Alveolar lung model

Alveolar lung model with human microvascular lung endothelial cells and alveolar epithelial type I and II cells.

Starter Kit Assay Kit Chip only

10x SynALI chips -IMN2 linear (3 µm slits)

Accessories including tubing, clamps, needles and syringes

Pneumatic priming device (required for priming tubing to remove air)

10x SynALI chips - IMN2 linear (3 µm slits)

Accessories including tubing, clamps, needles and syringes

3x SynALI chip - IMN2 linear (3 µm slits)

 

Chip size 75 mm x 25 mm
Outer channels width 200 µm
Central channel width 500 µm
Channel height 100 µm
Travel width 50 µm
Pore size 3 µm

Organ-on-a-Chip - Air Liquid Interface Lung Model_darwin microfluidics

The Airway model can be created with a co-culture of endothelial and epithelial cells and an Alveolar tri-culture model with endothelial, epithelial and fibroblasts is possible as well.

3D tumor manual

Air Liquid Interface model technical manual

These co-culture protocols have been developed to establish true vascular monolayers in communication with the 3D lung tissue. Human cells grown in these chips retain a biological phenotype similar to that found in real tissue. Leading researchers have validated that cells grown in these chips more accurately reflect the cell behavior found in vivo compared to cells grown using conventional culture techniques.

Unlike well-plate tests performed under static conditions, these chips reproduce the realistic dynamic conditions for the assessment of cell-drug and cell-cell interactions thereby providing an accurate in vitro platform to study and elucidate the mechanisms of success and failure. Compared to in vivo animal studies, they allow real-time visualization and analysis of the assay in a controlled environment.

Epi- and endothelial co-culture on SynALI chip

Epithelial and Endothelial Co-Culture in Microfluidic Chip. (a) and (b) Phase Contrast image of the confluent co-cultured chip with epithelial cells in the center channel and endothelial cells lining the peripheral channels. (c) Phase Contrast of top and bottom of the channel showing confluent monolayers in tissue and vascular channel. These images show the confluence of cells on both the top and bottom of the center channel and one side channel with a central, clear lumen. (d) Cross-sectional, 3-D reconstructed confocal image of co-cultured chip showing lumen formation in all three channels (10X mag.). Taken from Liu et al 2019.

 

📄SynALI technical manual

📄SynVivo brochure

Liu, Z., Mackay, S., Gordon, D. M., Anderson, J. D., Haithcock, D. W., Garson, C. J., ... & Guimbellot, J. S. (2019). Co-cultured microfluidic model of the airway optimized for microscopy and micro-optical coherence tomography imaging. Biomedical Optics Express, 10(10), 5414-5430. https://doi.org/10.1364/BOE.10.005414

 

Payment Terms

You can either order and pay via credit card or invoice (through a purchase order). Request a quote easily by clicking on the "Request a Quote" button in the cart page.

Please note that some products are not available for direct purchase: just click "Request a Quote" on the product page and fill in the form to get more informations!

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  • That's it! You will receive an order confirmation within a few minutes, and our team will send you the invoice.

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  • In the website header, click on Request a Quote
  • Fill in the required informations, then click on Submit Quote
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  • After receiving the quote, just send your purchase order at contact@darwin-microfluidics.com
  • Our team will complete your purchase and send you the invoice!

 

If you wish to pay in USD, GBP or AUD, please specify it in your PO. We can add the converted value in the required currency to the quote if requested.

 

Worldwide shipping

We ship worldwide from our warehouse in France.

Shipping charges are calculated based on the shipping country, and can be estimated from the cart page. Our shipping rates and times are:

  • France: GLS (1-2 business days), free shipping above 350€
  • Germany, Austria, Belgium, Denmark, Spain, Hungary, Italy, Luxembourg, Netherlands, Poland, Portugal, Czechia, Slovakia:
    • GLS (2-4 business days), free shipping above 350€
    • DHL Express (1-2 business days), reduced rate above 350€
  • Bulgaria, Croatia, Estonia, Finland, Ireland, Latvia, Lithuania, Romania, Slovenia, Sweden, Greece, Cyprus, Malta: DHL Express (1-2 business days), free shipping above 400€
  • United Kingdom: DHL Express (1-2 business days), free shipping above 400€
  • USA, Canada: DHL Express (1-3 business days), free shipping above 400€
  • Switzerland: DHL Express (1-2 days), free shipping above 450€
  • Other countries: DHL Express, shipping rates based on the destination, weight and dimensions

 

If you wish to use your own shipping account, we will charge a flat fee of 15€.

We reserve the right to apply a surcharge for parcels > 30 kg, palletized shipments, or packages with dimensions exceeding the maximum dimensions of our carriers.

Please note that our standard shipping incoterms are DAP (Delivered At Place), or EXW if using your own carrier.

Contact us if you have any questions!

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