NETRI - DuaLink MEA Organ-on-a-Chip Platform
The DuaLink NeoBento™ features 8 chips per plate. The DuaLink architecture has been specially designed to create fluidic isolation and co-culture of up to 2 cell types. The plate features a MicroElectrod Array (MEA) layer to record the activity of the neurons. Both channels are connected by microchannels to allow 2 independant cell cultures. Neurons are used as bio-sensors, cell bodies growing in one channel and extensions in microchannels to reach and innervate any organs cultivated in the opposite channel. Recording and processing of electrophysiological data is made possible via MEA technology and NETRI's UpLink utility software.
The NeuroFluidics MEA Line by NETRI is fabricated in PDMS for high transparency and biocompatibility and is compatible with MEA-recording (Axion Maestro EDGE). This technology is ideal for developing high-throughput organ-on-a-chip models to accelerate drug development and disease modeling.
General
NETRI’s NeuroFluidics MEA Devices are MEA-capable compartmentalized organ-on-chip. They are innovative organ-on-a-chip platforms for organ modeling and drug discovery. They offer high-throughput data collection, including electrophysiology, with up to 16 data points on the same plate With their pump-free design and standard ANSI 96-well plate format, they can be seamlessly integrated with standard lab equipment and are compatible with a wide range of readouts for in-depth data analysis. NETRI’s platforms aim to model a wide range of organs and use neurons as bio-sensors to innervate those organs.
The DuaLink design of the NeuroFluidics Devices allows the culture of up to 2 cell types and their electrical recording. It features 2 cell culture compartments each having its own media and coating solutions, connected through 1 compartment for fluidic isolation. Each of the 3 channels has one inlet and one outlet. In the DuaLink architecture, the lateral channels (1 and 3) are symmetric and the central channel (2) is smaller to create a fluidic isolation compartment between them, enabling interaction while isolating the cellular compartment. Because of the micron scale of microchannels connecting channels, only cell extensions can develop in the microchannels, while cell bodies remain in the cell compartment (channels 1 and 3).
Thanks to the MicroElectrode Array (MEA) plate, develop in collaboration with Axion BioSystems, it is possible to record the electrical impact of compound on neurons somas or extensions. With 42 electrodes (white dots in the picture below) displayed both in the channels and microchannels, it is possible to record all neurons' parts: somas, axons and dendrites. It is also possible to monitor the propagation of an action potential from one channel to the other.
The electrophysiological recordings are performed thanks to Axion Biosystems' Maestro EDGE (8 chips per plate) instruments and results can be processed thanks to NETRI's Uplink software for easy interpretation of the collected data.
The plate comes in the EDGE format, displaying 8 independent designs (half-plate).
This technology is compatible with a wide range of readouts including ELISA, Live Staining, ImmunoFluorescence, Live/Dead Assays, Electrophysiology and more.
The DuaLink MEA platform can be used for compartmentalization & electrical activity recording, to model CIPN, to model neuromuscular junction or to analyze the functional impact of compound on organ via neurons electrical activity.
Content
1x DuaLink MEA EDGE
Specifications
| Parameters | Channel 1 | Channel 2 | Channel 3 |
| Length (µm) | 18 800 | 6000 | 18 800 |
| Width (µm) | 1 000 | 200 | 1 000 |
| Height (µm) | 200 | 200 | 200 |
| Volume (µL) | 3.8 | 0.24 | 3.8 |
| Volume with inlets/outlets (µm) | 117.7 | 114.1 | 117.7 |
| Surface area (mm²) | 18.8 | 1.2 | 18.8 |
| Surface area with inlets/outlets (mm²) | 32.9 | 15.3 | 32.9 |
| Microchannels | 125x6(±1)x3,2 µm ; LxWxH n=200; spaced by 20 µm | ||
| Material | Chip in PDMS, Structure in Polystyrene | ||
| Format | Standard ANSI 96-well plate | ||
| Shel-life | 4 months after reception | ||
Documentation
DuaLink MEA technical specifications
DuaLink application note - Improve reproducibility
DuaLink application note - Surface tension-based cell seeding
DuaLink MEA application note - Compartmentalized culture of primary or hiPSC-derived neurons
RELATED PRODUCTS
The DuaLink NeoBento™ features 8 chips per plate. The DuaLink architecture has been specially designed to create fluidic isolation and co-culture of up to 2 cell types. The plate features a MicroElectrod Array (MEA) layer to record the activity of the neurons. Both channels are connected by microchannels to allow 2 independant cell cultures. Neurons are used as bio-sensors, cell bodies growing in one channel and extensions in microchannels to reach and innervate any organs cultivated in the opposite channel. Recording and processing of electrophysiological data is made possible via MEA technology and NETRI's UpLink utility software.
The NeuroFluidics MEA Line by NETRI is fabricated in PDMS for high transparency and biocompatibility and is compatible with MEA-recording (Axion Maestro EDGE). This technology is ideal for developing high-throughput organ-on-a-chip models to accelerate drug development and disease modeling.
General
NETRI’s NeuroFluidics MEA Devices are MEA-capable compartmentalized organ-on-chip. They are innovative organ-on-a-chip platforms for organ modeling and drug discovery. They offer high-throughput data collection, including electrophysiology, with up to 16 data points on the same plate With their pump-free design and standard ANSI 96-well plate format, they can be seamlessly integrated with standard lab equipment and are compatible with a wide range of readouts for in-depth data analysis. NETRI’s platforms aim to model a wide range of organs and use neurons as bio-sensors to innervate those organs.
The DuaLink design of the NeuroFluidics Devices allows the culture of up to 2 cell types and their electrical recording. It features 2 cell culture compartments each having its own media and coating solutions, connected through 1 compartment for fluidic isolation. Each of the 3 channels has one inlet and one outlet. In the DuaLink architecture, the lateral channels (1 and 3) are symmetric and the central channel (2) is smaller to create a fluidic isolation compartment between them, enabling interaction while isolating the cellular compartment. Because of the micron scale of microchannels connecting channels, only cell extensions can develop in the microchannels, while cell bodies remain in the cell compartment (channels 1 and 3).
Thanks to the MicroElectrode Array (MEA) plate, develop in collaboration with Axion BioSystems, it is possible to record the electrical impact of compound on neurons somas or extensions. With 42 electrodes (white dots in the picture below) displayed both in the channels and microchannels, it is possible to record all neurons' parts: somas, axons and dendrites. It is also possible to monitor the propagation of an action potential from one channel to the other.
The electrophysiological recordings are performed thanks to Axion Biosystems' Maestro EDGE (8 chips per plate) instruments and results can be processed thanks to NETRI's Uplink software for easy interpretation of the collected data.
The plate comes in the EDGE format, displaying 8 independent designs (half-plate).
This technology is compatible with a wide range of readouts including ELISA, Live Staining, ImmunoFluorescence, Live/Dead Assays, Electrophysiology and more.
The DuaLink MEA platform can be used for compartmentalization & electrical activity recording, to model CIPN, to model neuromuscular junction or to analyze the functional impact of compound on organ via neurons electrical activity.
Content
1x DuaLink MEA EDGE
Specifications
| Parameters | Channel 1 | Channel 2 | Channel 3 |
| Length (µm) | 18 800 | 6000 | 18 800 |
| Width (µm) | 1 000 | 200 | 1 000 |
| Height (µm) | 200 | 200 | 200 |
| Volume (µL) | 3.8 | 0.24 | 3.8 |
| Volume with inlets/outlets (µm) | 117.7 | 114.1 | 117.7 |
| Surface area (mm²) | 18.8 | 1.2 | 18.8 |
| Surface area with inlets/outlets (mm²) | 32.9 | 15.3 | 32.9 |
| Microchannels | 125x6(±1)x3,2 µm ; LxWxH n=200; spaced by 20 µm | ||
| Material | Chip in PDMS, Structure in Polystyrene | ||
| Format | Standard ANSI 96-well plate | ||
| Shel-life | 4 months after reception | ||
Documentation
DuaLink MEA technical specifications
DuaLink application note - Improve reproducibility
DuaLink application note - Surface tension-based cell seeding
DuaLink MEA application note - Compartmentalized culture of primary or hiPSC-derived neurons