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HUMIMIC Chip3 & Chip3plus - 3-organ-chip

The HUMIMIC Chip3 and Chip3plus are cutting-edge organ-on-a-chip platforms allowing the simultaneous culture of three tissue types in a single microfluidic circuit. It provides a more realistic and dynamic model of human physiology. This 3-organ platform enables the study of complex inter-organ interactions, drug responses, and disease mechanisms in a controlled, miniature environment.
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General

The HUMIMIC Chip3 and Chip3plus allow the co-culture of up to 3 different tissues in a joint microfluidic circuit to develop Microphysiological Systems (MPS). The HUMIMIC Chips are operated via the HUMIMIC Starter, which can control several chips and chip types at the same time. Pressure and vacuum can be applied and allow the modeling of a lifelike supply of the tissues and also the modulation of shear stress according to the experimental needs of the user. The HUMIMIC Chip2 contains 3 pump membranes (500 µm thick) to control the liquid displacement in the microfluidic circuit, through valves activated by the pressure applied by the HUMIMIC Starter.

The flexibility of the HUMIMIC device allows to study any organ and also biological barrier models like the intestine, the lung or the skin. It is compatible with cell line, primary cells but also biopsies, tissue sections or spheroids. It is also possible to use commercially available culture inserts or even matrix-suported cultures to mimick the 3D environment of the organs. These chips, made of polycarbonate, PDMS and glass, have been designed for long-term experiments allowing the study of disease models and drug's efficacy and toxicity.

The Chip3 devices exists in 3 formats, displaying different sizes of culture compartment to adapt to the studied organs and tissue types:

  • The Chip3 displaying two "96-well" (6.5 mm of diameter) and one bigger "24-well" (14 mm of diameter)
  • The Chip3plus with one "96-well" and two "24-well"
  • Here we propose two different versions of the Chip3plus, one for regular tissue studies and one to mimick air-liquid interfaces (Chip3plus-ALI).

Each chip is provided with the relevant culture compartments which are screwed into the device thanks to a hexagonal socket wrench. For media exchange, the compartments of one circuit need to be opened separately.

The types of culture compartment proposed for each chip are described in the specification section. Note that the nature of the culture compartment can be changed according to what is described in the product catalog of the Documentation section.

All chips are provided prefilled with DPBS supplemented with 1% Penicillin/Streptomycin (PS) but can alos be provided with a mix of 0.01% Gentamicin / 0.1% Amphotericin B (GA) or without any antibiotics (WO) added in the DPBS. For any requests regarding customization, please contact us. 

Content

1x HUMIMIC Chip3 or Chip3plus (including cell culture compartments)

Specifications
  Chip3 Chip2 96-well
Culture compartments One 24-well & two 96-well Two 24-well & one 96-well
Microfluidic volume  10 µL 11 µL
Microfluidic surface 235 mm² 255 mm²
Channel height  100 µm
Channel width 500 µm
Adapter plate material Polycarbonate
Microfluidic layer material PDMS
 Microscopic slide material Glass (clear, ISO8037/1)
Culture compartments material  PEEK, Polycarbonate
 Sealings material MVQ 70A
Storage temperature 2 - 8 °C
  • Chip3:
    • 1x Threadfit 01: compatible with Transwell inserts or medium reservoir of up to 350 µL
    • 1x Threadfit 03: compatible with scaffolds or medium reservoir of up to 750µL 
    • 1x Threadfit 07: compatible with Transwell inserts 
  • Chip3plus: 
    • 1x Threadfit 03: compatible with scaffolds or medium reservoir of up to 750µL 
    • 1x Threadfit 07: compatible with Transwell inserts  
    • 1x Threadfit 08: medium reservoir of up to 3 mL 
  • Chip3plus - ALI: 
    • 1x Threadfit 03: compatible with scaffolds or medium reservoir of up to 750µL 
    • 1x Threadfit 07: compatible with Transwell inserts 
    • 1x Airfit 06: compatible with Transwell inserts, with airpermeable lid, recommended for air exposed tissue models. Can only be used once per circuit.

Technical drawing of the Chip3

Chip3 technical drawing

Technical drawing of the Chip3plus

Chip3plus technical drawing

 

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Darwin Microfluidics being based in France, ships orders under Delivered At Place (DAP) incoterms. This means that for customers outside the European Union (EU), you are responsible for any applicable customs fees, duties, and taxes in addition to the shipping costs. These charges are determined by your local customs authority and must be paid by you upon the arrival of the shipment in your country.

We recommend contacting your local customs office for detailed information on these charges if you're ordering from outside the EU, as they can vary depending on the destination and the value of your order. Please note that these additional costs are not included in the shipping fees charged by Darwin Microfluidics.

If you've received your order from Darwin Microfluidics and found that a product is defective or missing, please follow these steps to resolve the issue:

Report immediately: Contact our customer service team as soon as possible. Reporting the issue promptly helps us address your concern more efficiently.

Provide details: When you contact us, please provide your order number, the name the order was placed under, and a detailed description of the issue. For defective products, include clear photos or a video that shows the defect.

Review: Our team will review your claim to determine the best course of action. We may ask for additional information or details about the problem.

Resolution: Depending on the situation, we may send a replacement for the defective or missing item, provide a refund, or offer another satisfactory resolution. Our goal is to ensure you are completely satisfied with your purchase.

Follow-up: We will keep you updated throughout the process and confirm when the issue has been resolved to your satisfaction.

We apologize for any inconvenience caused by a defective or missing item and are committed to resolving your issue as quickly and smoothly as possible.

At Darwin Microfluidics, our goal is to offer the most competitive prices directly as our list price, ensuring that all our customers receive great value without needing to navigate complex discount structures. Here’s a detailed look at our discount policy:

Competitive list prices: We work hard to ensure our list prices are among the most competitive in the market. This approach allows us to offer fair pricing to all our customers from the start, eliminating the need for frequent discounts.

Quantity-based discounts: For certain products, we offer quantity-based discounts directly on the product pages. These discounts are automatically applied based on the quantity of the product you purchase, providing savings for bulk purchases without the need for special codes or negotiations.

Bulk orders: While we strive to offer the best prices upfront, we understand that customers making large-scale purchases may have special considerations. In such cases, we invite you to discuss your needs with our sales team. Depending on the size and nature of your order, we may be able to offer tailored discounts or pricing adjustments.

We believe in transparency and simplicity in pricing, allowing you to make your purchases with confidence, knowing you're receiving a fair deal without the hassle of negotiating discounts. If you have any questions about pricing or discounts for bulk orders, please don't hesitate to reach out to our sales team for personalized assistance.

Please follow our returns & refund page.

Since Darwin Microfluidics sells exclusively to companies and institutions and is based in France, the VAT treatment for your orders depends on the location of your company or institution:

Within the EU (excluding France): For customers in other EU countries, the reverse charge mechanism applies. This means that VAT is not charged by us at the point of sale. Instead, it is your responsibility to account for the VAT at the standard rate applicable in your country under the reverse charge procedure. This process helps simplify cross-border transactions within the EU.

In France: For customers located in France, standard French VAT rates apply to all purchases. As a France-based company, we are required to charge VAT on sales to other French companies and institutions, which you can then reclaim according to local VAT procedures if applicable.

Outside the EU: For customers outside the European Union, sales are not subject to VAT by us. However, you may be subject to import duties, taxes, and charges that are not included in the item price or shipping cost. These charges are the buyer's responsibility and vary by country. We advise checking with your local customs office for more information.

Please ensure your VAT identification number is provided during the ordering process for EU transactions to qualify for the reverse charge mechanism. If you have any questions regarding VAT charges or need further assistance with VAT-related issues, please contact our customer service team.

We are unable to provide specific estimates for customs fees, duties, or taxes associated with your order. These charges are determined by the customs authority in your country and can vary based on a variety of factors, including the type of items purchased, their value, and the country to which they are being shipped.

We recommend contacting your local customs office directly to get the most accurate and up-to-date information regarding potential customs charges for your order. They can provide you with detailed guidelines on the fees you might expect to pay based on the products you're importing from us.

Please remember, these charges are the responsibility of the recipient and are not included in the shipping costs paid to Darwin Microfluidics.

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