This system is recommended for easy and large-scale production of 3D cell models such as spheroids, organoids and microtissues. It is based on hanging-drop technique for spheroid formation and combined with SureXchange unique well design for long-term spheroid culture, analysis and observation.
General
The Akura™ PLUS Hanging Drop System is a 2-plate system: one Akura™ PLUS Hanging Drop plate and one Akura™ 96 Spheroid Microplate. It allows the robust formation of spheroids, and their long-term growth, culturing, and analysis thanks to available protocols.
The formation of spheroids starts with the delivery of a cell suspension (40 µL) in the SureDrop™ inlet funnels of the PLUS plate with a pipette or automated liquid handler. Hanging drops will then form at the bottom and spheroids will be formed by gravity-assisted assembly within 2-4 days.
During spheroid formation, the initial cell suspension should be homogenous for getting reproducible results. To prevent the evaporation of hanging drops, a humidifier pad is included in the system and should be put in the bottom tray of the PLUS plate to ensure optimal humidity.
Once spheroids are constituted, they can be transferred to the Akura™ 96 plate for culturing or downstream analysis. This 96-well plate presents a unique SureXchange well design which allows near complete medium exchange without spheroid loss. These plates are compatible with most laboratory equipment thanks to their ANSI/SLAS format, a 1 mm-observation window for easy imaging, and simple medium collection for biochemical assays. For more details on this plate, take a look at the dedicated product page of Akura™ 96 Spheroid Microplate.
Content
10x Akura™ PLUS Hanging Drop plates including bottom plate with reservoir, plate, lid and humidifier pad.
10x Akura™ 96 Spheroid Microplates
Specifications
The Akura™ Hanging Drop plate consists of a bottom plate with a reservoir, a frame with 12x8 well strips, a lid, and a humidifier pad.
| Plate length |
127.76 mm |
| Plate width |
85.48 mm |
| Height of plate |
22.51 mm |
| Well diameter (top) |
5.8 mm |
| Well-to-well distance |
9.0 mm |
| Working volume |
40 µL |
| Material |
Polystyrene |
The Akura™ 96 Spheroid Microplate meets the SLAS/ANSI standards with alphanumeric labeling of 96 wells arranged in 8 rows and 12 columns.
| Plate length |
127.76 mm |
| Plate width |
85.48 mm |
| Height of plate |
14.35 mm |
| Height of plate with lid |
15.35 mm |
| Height of well |
12.75 mm |
| Skirt height |
0.4 mm |
| Diameter well opening |
6.70 mm |
| Diameter well bottom |
1 mm |
| Thickness well bottom |
0.8 mm |
| Working volume |
70-80 µL |
| Well-to-well distance |
9 mm |
| SureXchange tip position |
1.71 mm horizontal offset, 9.86 in z-height
|
| Plate and lid material |
COP (Cyclo-olefin-polymer), Polystyrene
|
Documentation
? Akura PLUS Hanging Drop System - Product Manual
? Akura PLUS Hanging Drop System - Quick Start Guide
? Spheroid Aggregation Protocol with Akura PLUS Hanging Drop System
? Akura PLUS Hanging Drop System - Technical specification
