The Akura™ 96-well plate is an appropriate tool for the generation, long-term cultivation, and analysis of 3D cell models. The unique well design ensures high reproducibility, scalability, and near-complete medium exchange without spheroid loss. The plate is made of COP (cyclic olefin polymer) and its format is compliant with SLAS standards for easy integration in any laboratory.
General
Thanks to its unique conical well design, this 96-well plate enables scaffold-free aggregation and long-term culturing of 3D cell models while being compatible with a broad range of endpoints. The plate supports several 3D cell models such as spheroids, organoids, and microtissues of up to 750 µm. The ultra-low attachment (ULA) wells’ coating prevents cell attachment to the surface and enhances spheroid formation.
Spheroid formation and maturation appear within 2-5 days of incubation at 37°C, following cell seeding and brief centrifugation. The maturation process can be improved by tilting the plate to 30° or placing it on the Akura™ Tilting Stand.
The SureXchange unique well design has been developed to ensure near-complete (>90%) medium exchange without spheroid loss due to unintended aspiration.
This technology shows great reproducibility and uniformity in spheroid formation and allows for long-term culturing. It also helps improve washing steps to remove unwanted components (medium components, hormones) which could interfere with endpoint assays or spheroid behavior.
With an ANSI/SLAS standard format, this plate is compatible with all existing laboratory equipment, including multi-channel pipettes and automated liquid handling systems for higher throughput.
The plate is made of COP (cyclo-olefin-polymer) presenting high optical transparency and allowing the plate to be used with most high-content imaging platforms. Easy visualization of spheroids is achieved thanks to the 1 mm diameter flat bottom (0.8mm thick) observation window.
Content
IS-CS-PB13: 5x Akura™ 96 Spheroid Microplates (with lids)
IS-CS-PB15: 20x Akura™ 96 Spheroid Microplates (with lids)
Specifications
The Akura™ 96 Spheroid Microplate meets the SLAS/ANSI standards with alphanumeric labeling of 96 wells arranged in 8 rows and 12 columns.
| Plate length |
127.76 mm |
| Plate width |
85.48 mm |
| Height of plate |
14.35 mm |
| Height of plate with lid |
15.35 mm |
| Height of well |
12.75 mm |
| Skirt height |
0.4 mm |
| Diameter well opening |
6.70 mm |
| Diameter well bottom |
1 mm |
| Thickness well bottom |
0.8 mm |
| Working volume |
70-80 µL |
| Well-to-well distance |
9 mm |
| SureXchange tip position |
1.71 mm horizontal offset, 9.86 in z-height
|
| Plate and lid material |
COP (Cyclo-olefin-polymer), Polystyrene
|
Documentation
? Akura 96 Microplate - Product Manual
? Akura 96 Microplate - Quick Start Guide
? Akura Spheroid Aggregation Protocol
? Akura 96 Microplate - Technical specification
