Organ-on-a-Chip - Cross-flow membrane - Mini Luer
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Organ-on-a-Chip - Cross-flow membrane - Mini Luer

This convenient and affordable Organ-on-a-Chip is a compact solution to recreate biological barriers in vitro. One chip hosts two different culture chambers and each of these chambers allows the co-culture of different cell types within two independent compartments divided by a permeable membrane.
The chip is fabricated in Topas (COC, cyclic olefin copolymer) for better light transmittance. The inlets and outlets with integrated Mini Luer connections ensure leak-free junctions with the tubing and allow experiments under dynamic flow conditions.
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€79.50
This convenient and affordable Organ-on-a-Chip is a compact solution to recreate biological barriers in vitro. One chip hosts two different culture chambers and each of these chambers allows the co-culture of different cell types within two independent compartments divided by a permeable membrane.
The chip is fabricated in Topas (COC, cyclic olefin copolymer) for better light transmittance. The inlets and outlets with integrated Mini Luer connections ensure leak-free junctions with the tubing and allow experiments under dynamic flow conditions.

This Organ-on-a-Chip with a cross-flow membrane allows you to reliably mimic physiological conditions in vitro and study tissue organization, cell-cell interactions, barrier penetration, and physiological responses in a more in vivo-like environment.

Two different and independent culture chambers are available on the same chip. This allows you to perform two experiments in parallel or in series (body-on-a-chip).

The two chambers are designed to develop models of physiological barriers and to ensure an in vivo-like environment under static or dynamic flow conditions. Each chamber indeed has 2 flow inlets and 2 outlets for the perfusion of culture media and testing solutions in both the apical and basolateral compartments.

The entire body of the chip is made of Topas, a cyclic olefin copolymer (COC) to overcome the limitations of standard PDMS. Topas does not unspecifically absorb medium contents and has high stability and ideal optical characteristics for the bright field as well as fluorescence microscopy. Topas is frequently used in medical devices due to its proven biocompatibility.

The chip comes with two options of PET membranes having different pore sizes. Choose between a 0.2 µm porous membrane or an 8 µm porous membrane.

Features

  • Mini Luer connection molded with the chip: leak-free
  • Highly resistant and optically clear material: Topas (COC, cyclic olefin copolymer) has high transparency (equivalent to glass) and very low grade of auto-fluorescence
  • Standard microscope glass slide size (75.5 mm x 25.5 mm x 1.5 mm)
  • Cover lid thickness: 140 µm
  • 2 independent cell culture chambers
  • Porous PET membrane: pore Φ of 0.2 or 8 µm

 

Cross-flow porous membrane Upper/Apical compartment Bottom/Basolateral compartment

Membrane of the cross-flow membrane organ-on-a-chip

Upper compartment of the cross-flow membrane organ-on-a-chip Bottom compartment of the cross-flow membrane organ-on-a-chip

Applications and examples from published papers

The upper and the lower compartments are separated by the porous membrane, and they can be perfused with different culture media. A tissue interface can be created to mimic alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc. Tissues inside the chip can be easily observed by microscopy.

Cell culture is just one potential application area of this versatile chip. The design indeed allows other different experiments such as small molecule transfer measurements, on-chip dialysis, and many more.

Intestine-on-chip

Maurer M. et al., A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies, Biomaterials 2019 (Download)

Intestine-on-chip images from the linked article

3D microphysiological model of the human intestine.

In this work, the model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonized with living microorganisms. The authors demonstrate that microbial interactions can be efficiently investigated using this chip creating a more physiological and immunocompetent microenvironment.

Endothelial Barrier-on-chip

Raasch M. et al., Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions, Biofabrication 2015, 7: 015013 (Download)

Endothelial cells layers images from the linked article

The authors investigated cell viability, expression of endothelial markers, and cell adhesion molecules of ECs dynamically cultured under low and high shear stress. This chip allows an effective supply with nutrition medium, discharge of catabolic cell metabolites, and defined application of shear stress to ECs under laminar flow conditions.

They show that ECs cultured in the chip form a tight EC monolayer with increased cellular density and enhanced cell layer thickness compared to static and two-dimensionally perfused cell culture conditions. Endothelial layers in the chip express higher amounts of EC marker proteins von-Willebrand-factor and PECAM-1.

Liver-on-chip

Rennert K. et al., A microfluidically perfused three dimensional human liver model, Biomaterials 2015, 119-131 (Download)

Liver-on-chip images from the linked article

The microfluidically perfused chip enables sufficient nutrition supply and resembles morphological aspects of the human liver sinusoid. It utilizes a suspended membrane as a cell substrate mimicking the space of Disse and the perfusion enhances the formation of hepatocyte microvilli. The authors stated that the perfused liver chip shares relevant morphological and functional characteristics with the human liver and represents a new in vitro research tool to study human hepatocellular physiology at the cellular level under conditions close to the physiological situation.

During culture in the biochip HepaRG cells consistently differentiate into cells exhibiting a hepatocyte phenotype and into cells with biliary epithelial cell phenotype that self-organize into a hepatocyte layer with functional bile ducts.

1x Cross-flow membrane organ-on-a-chip with mini Luer ports

Please use this information to precisely define the key parameters of your cell seeding and experimental protocols. One chip allows you to perform two independent experiments.


Upper compartment Bottom compartment
Volume (µL) 87.5 61.5
Total surface (mm²) 440 271
Ground surface (mm²) 154 118
Chamber height (µm) 700 400
Width afferent channel (mm) 0.8
Width efferent channel (mm) 2
Height afferent channel (mm) 0.6
Height efferent channel (mm) 0.4

 

Porous membrane
Area of interaction (mm²) 36
Pore size (µm) 0.2 8
Pore density (pores/cm²) 5×10^8 1×10^5
Cell culture area (cm²) 1.1
Thickness (µm) 12
Material polyethylene terephthalate (PET)

Dimensions of the cross-flow membrane organ-on-a-chip

Click to read more information about ChipShop chips material properties.

Maurer, M., Gresnigt, M. S., Last, A., Wollny, T., Berlinghof, F., Pospich, R., ... & Mosig, A. S. (2019). A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies. Biomaterials,220, 119396. https://doi.org/10.1016/j.biomaterials.2019.119396

Raasch, M., Rennert, K., Jahn, T., Peters, S., Henkel, T., Huber, O., ... & Mosig, A. (2015). Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions. Biofabrication, 7(1), 015013. doi:10.1088/1758-5090/7/1/015013

Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A. M., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., Peters, F. T., Lupp, A., Gärtner, C., Mayr, T., Bauer, M., Huber, O., & Mosig, A. S. (2015). A microfluidically perfused three dimensional human liver model. Biomaterials, 71, 119–131. https://doi.org/10.1016/j.biomaterials.2015.08.043

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