Organ-on-a-Chip - Cross-flow membrane - Luer and Mini Luer
The Fluidic 568 Organ-on-a-Chip by Microfluidic ChipShop is a cost-effective and compact solution for recreating in vitro biological barriers. Each chip features two distinct culture chambers, allowing the co-culture of various cell types in two independent compartments separated by a permeable membrane.
The chip is fabricated in Topas (COC, cyclic olefin copolymer) for better light transmittance. Half of the ports on this chip are integrated with Luer connection ports to interface fluidic reservoirs. The remaining ports are mini Luer interfaces.
General
This Organ-on-a-Chip with a cross-flow membrane allows you to reliably mimic physiological conditions in vitro and study tissue organization, cell-cell interactions, barrier penetration, and physiological responses in a more in vivo-like environment.
Two different and independent culture chambers are available on the same chip. This allows you to perform two experiments in parallel or in series (body-on-a-chip).
The two chambers are designed to develop models of physiological barriers and to ensure an in vivo-like environment under static or dynamic flow conditions. Each chamber indeed has 2 flow inlets and 2 outlets for the perfusion of culture media and testing solutions in both the apical and basolateral compartments.
The entire body of the chip is made of Topas, a cyclic olefin copolymer (COC) to overcome the limitations of standard PDMS. Topas does not unspecifically absorb medium contents and has high stability and ideal optical characteristics for the bright field as well as fluorescence microscopy. Topas is frequently used in medical devices due to its proven biocompatibility.
The chip comes with an 8 µm porous membrane. Each chamber has 2 Luer inlets to interface fluidic reservoirs and 2 mini Luer outlets.
Features
- Luer and mini Luer connection molded with the chip: leak-free
- Highly resistant and optically clear material: Topas (COC, cyclic olefin copolymer) has high transparency (equivalent to glass) and very low grade of auto-fluorescence
- Standard microscope glass slide size (75.5 mm x 25.5 mm x 1.5 mm)
- Cover lid thickness: 140 µm
- 2 independent cell culture chambers
- 8 µm porous PET membrane
| Cross-flow porous membrane | Upper/Apical compartment | Bottom/Basolateral compartment |
|
|
 |
 |
Applications and examples from published papers
The upper and the lower compartments are separated by the porous membrane, and they can be perfused with different culture media. A tissue interface can be created to mimic alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc. Tissues inside the chip can be easily observed by microscopy.
Cell culture is just one potential application area of this versatile chip. The design indeed allows other different experiments such as small molecule transfer measurements, on-chip dialysis, and many more.
Intestine-on-chip
Maurer M. et al., A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies, Biomaterials 2019 (Download)
3D microphysiological model of the human intestine.
In this work, the model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonized with living microorganisms. The authors demonstrate that microbial interactions can be efficiently investigated using this chip creating a more physiological and immunocompetent microenvironment.
Endothelial Barrier-on-chip
Raasch M. et al., Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions, Biofabrication 2015, 7: 015013 (Download)
The authors investigated cell viability, expression of endothelial markers, and cell adhesion molecules of ECs dynamically cultured under low and high shear stress. This chip allows an effective supply with nutrition medium, discharge of catabolic cell metabolites, and defined application of shear stress to ECs under laminar flow conditions.
They show that ECs cultured in the chip form a tight EC monolayer with increased cellular density and enhanced cell layer thickness compared to static and two-dimensionally perfused cell culture conditions. Endothelial layers in the chip express higher amounts of EC marker proteins von-Willebrand-factor and PECAM-1.
Liver-on-chip
Rennert K. et al., A microfluidically perfused three dimensional human liver model, Biomaterials 2015, 119-131 (Download)
The microfluidically perfused chip enables sufficient nutrition supply and resembles morphological aspects of the human liver sinusoid. It utilizes a suspended membrane as a cell substrate mimicking the space of Disse and the perfusion enhances the formation of hepatocyte microvilli. The authors stated that the perfused liver chip shares relevant morphological and functional characteristics with the human liver and represents a new in vitro research tool to study human hepatocellular physiology at the cellular level under conditions close to the physiological situation.
During culture in the biochip HepaRG cells consistently differentiate into cells exhibiting a hepatocyte phenotype and into cells with biliary epithelial cell phenotype that self-organize into a hepatocyte layer with functional bile ducts.
Content
1x Cross-flow membrane organ-on-a-chip with Luer and mini Luer ports
Specifications
Please use this information to precisely define the key parameters of your cell seeding and experimental protocols. One chip allows you to perform two independent experiments.
| Upper compartment | Bottom compartment | |
| Volume (µL) | 105.8 | 64 |
| Total surface (mm²) | 605 | 281 |
| Ground surface (mm²) | 151 | 120 |
| Pore size (µm) | 8 | |
| Lid thickness (µm) | 140 | |
| Porous membrane | |
| Area of interaction (mm²) | 40.6 |
| Pore size (µm) | 8 |
| Membrane reference | mcs-membrane 120 |
| Pore density (pores/cm²) | 1×10^5 +/- 0.02 |
| Thickness (µm) | 11.5 +/- 1.5 |
| Material | Hydrophilized polyethylene terephthalate (PET) |
| Color | Transparent |
| Pore orientation | Parallel, perpendicular to the surface |
| Imaging | Yes |
| Chip material | Topas (COC) |
| Lid thickness (µm) | 140 |
Documentation
Click to read more information about ChipShop chips material properties.
? General handling guide for cross-flow membrane chips
Maurer, M., Gresnigt, M. S., Last, A., Wollny, T., Berlinghof, F., Pospich, R., ... & Mosig, A. S. (2019). A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies. Biomaterials,220, 119396. https://doi.org/10.1016/j.biomaterials.2019.119396
Raasch, M., Rennert, K., Jahn, T., Peters, S., Henkel, T., Huber, O., ... & Mosig, A. (2015). Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions. Biofabrication, 7(1), 015013. doi:10.1088/1758-5090/7/1/015013
Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A. M., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., Peters, F. T., Lupp, A., Gärtner, C., Mayr, T., Bauer, M., Huber, O., & Mosig, A. S. (2015). A microfluidically perfused three dimensional human liver model. Biomaterials, 71, 119–131. https://doi.org/10.1016/j.biomaterials.2015.08.043
The Fluidic 568 Organ-on-a-Chip by Microfluidic ChipShop is a cost-effective and compact solution for recreating in vitro biological barriers. Each chip features two distinct culture chambers, allowing the co-culture of various cell types in two independent compartments separated by a permeable membrane.
The chip is fabricated in Topas (COC, cyclic olefin copolymer) for better light transmittance. Half of the ports on this chip are integrated with Luer connection ports to interface fluidic reservoirs. The remaining ports are mini Luer interfaces.
General
This Organ-on-a-Chip with a cross-flow membrane allows you to reliably mimic physiological conditions in vitro and study tissue organization, cell-cell interactions, barrier penetration, and physiological responses in a more in vivo-like environment.
Two different and independent culture chambers are available on the same chip. This allows you to perform two experiments in parallel or in series (body-on-a-chip).
The two chambers are designed to develop models of physiological barriers and to ensure an in vivo-like environment under static or dynamic flow conditions. Each chamber indeed has 2 flow inlets and 2 outlets for the perfusion of culture media and testing solutions in both the apical and basolateral compartments.
The entire body of the chip is made of Topas, a cyclic olefin copolymer (COC) to overcome the limitations of standard PDMS. Topas does not unspecifically absorb medium contents and has high stability and ideal optical characteristics for the bright field as well as fluorescence microscopy. Topas is frequently used in medical devices due to its proven biocompatibility.
The chip comes with an 8 µm porous membrane. Each chamber has 2 Luer inlets to interface fluidic reservoirs and 2 mini Luer outlets.
Features
- Luer and mini Luer connection molded with the chip: leak-free
- Highly resistant and optically clear material: Topas (COC, cyclic olefin copolymer) has high transparency (equivalent to glass) and very low grade of auto-fluorescence
- Standard microscope glass slide size (75.5 mm x 25.5 mm x 1.5 mm)
- Cover lid thickness: 140 µm
- 2 independent cell culture chambers
- 8 µm porous PET membrane
| Cross-flow porous membrane | Upper/Apical compartment | Bottom/Basolateral compartment |
|
|
|
|
Applications and examples from published papers
The upper and the lower compartments are separated by the porous membrane, and they can be perfused with different culture media. A tissue interface can be created to mimic alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc. Tissues inside the chip can be easily observed by microscopy.
Cell culture is just one potential application area of this versatile chip. The design indeed allows other different experiments such as small molecule transfer measurements, on-chip dialysis, and many more.
Intestine-on-chip
Maurer M. et al., A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies, Biomaterials 2019 (Download)
3D microphysiological model of the human intestine.
In this work, the model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonized with living microorganisms. The authors demonstrate that microbial interactions can be efficiently investigated using this chip creating a more physiological and immunocompetent microenvironment.
Endothelial Barrier-on-chip
Raasch M. et al., Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions, Biofabrication 2015, 7: 015013 (Download)
The authors investigated cell viability, expression of endothelial markers, and cell adhesion molecules of ECs dynamically cultured under low and high shear stress. This chip allows an effective supply with nutrition medium, discharge of catabolic cell metabolites, and defined application of shear stress to ECs under laminar flow conditions.
They show that ECs cultured in the chip form a tight EC monolayer with increased cellular density and enhanced cell layer thickness compared to static and two-dimensionally perfused cell culture conditions. Endothelial layers in the chip express higher amounts of EC marker proteins von-Willebrand-factor and PECAM-1.
Liver-on-chip
Rennert K. et al., A microfluidically perfused three dimensional human liver model, Biomaterials 2015, 119-131 (Download)
The microfluidically perfused chip enables sufficient nutrition supply and resembles morphological aspects of the human liver sinusoid. It utilizes a suspended membrane as a cell substrate mimicking the space of Disse and the perfusion enhances the formation of hepatocyte microvilli. The authors stated that the perfused liver chip shares relevant morphological and functional characteristics with the human liver and represents a new in vitro research tool to study human hepatocellular physiology at the cellular level under conditions close to the physiological situation.
During culture in the biochip HepaRG cells consistently differentiate into cells exhibiting a hepatocyte phenotype and into cells with biliary epithelial cell phenotype that self-organize into a hepatocyte layer with functional bile ducts.
Content
1x Cross-flow membrane organ-on-a-chip with Luer and mini Luer ports
Specifications
Please use this information to precisely define the key parameters of your cell seeding and experimental protocols. One chip allows you to perform two independent experiments.
| Upper compartment | Bottom compartment | |
| Volume (µL) | 105.8 | 64 |
| Total surface (mm²) | 605 | 281 |
| Ground surface (mm²) | 151 | 120 |
| Pore size (µm) | 8 | |
| Lid thickness (µm) | 140 | |
| Porous membrane | |
| Area of interaction (mm²) | 40.6 |
| Pore size (µm) | 8 |
| Membrane reference | mcs-membrane 120 |
| Pore density (pores/cm²) | 1×10^5 +/- 0.02 |
| Thickness (µm) | 11.5 +/- 1.5 |
| Material | Hydrophilized polyethylene terephthalate (PET) |
| Color | Transparent |
| Pore orientation | Parallel, perpendicular to the surface |
| Imaging | Yes |
| Chip material | Topas (COC) |
| Lid thickness (µm) | 140 |
Documentation
Click to read more information about ChipShop chips material properties.
? General handling guide for cross-flow membrane chips
Maurer, M., Gresnigt, M. S., Last, A., Wollny, T., Berlinghof, F., Pospich, R., ... & Mosig, A. S. (2019). A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies. Biomaterials,220, 119396. https://doi.org/10.1016/j.biomaterials.2019.119396
Raasch, M., Rennert, K., Jahn, T., Peters, S., Henkel, T., Huber, O., ... & Mosig, A. (2015). Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions. Biofabrication, 7(1), 015013. doi:10.1088/1758-5090/7/1/015013
Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A. M., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., Peters, F. T., Lupp, A., Gärtner, C., Mayr, T., Bauer, M., Huber, O., & Mosig, A. S. (2015). A microfluidically perfused three dimensional human liver model. Biomaterials, 71, 119–131. https://doi.org/10.1016/j.biomaterials.2015.08.043