Organ-on-a-Chip - Cross-flow membrane w/ Chamber Interaction - Mini Luer
Rated 3.5/5 based on 11 customer reviews

Organ-on-a-Chip - Cross-flow membrane w/ Chamber Interaction - Mini Luer

The Fluidic 747 Organ-on-a-Chip by Microfluidic ChipShop is a budget-friendly and compact solution for simulating in vitro biological barriers. Each chip contains two culture chambers connected via their upper compartments, with both chambers featuring two sections divided by a permeable membrane.
The chip is fabricated in Topas (COC, cyclic olefin copolymer) for better light transmittance. The inlets and outlets with integrated Mini Luer connections ensure leak-free junctions with the tubing and allow experiments under dynamic flow conditions.
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€79.50
The Fluidic 747 Organ-on-a-Chip by Microfluidic ChipShop is a budget-friendly and compact solution for simulating in vitro biological barriers. Each chip contains two culture chambers connected via their upper compartments, with both chambers featuring two sections divided by a permeable membrane.
The chip is fabricated in Topas (COC, cyclic olefin copolymer) for better light transmittance. The inlets and outlets with integrated Mini Luer connections ensure leak-free junctions with the tubing and allow experiments under dynamic flow conditions.

This Organ-on-a-Chip with a cross-flow membrane allows you to reliably mimic physiological conditions in vitro and study tissue organization, cell-cell interactions, barrier penetration, and physiological responses in a more in vivo-like environment.

Two connected culture chambers are available on the same chip, to perform two experiments in parallel or in series (body-on-a-chip) through the upper compartment connection.

This chip is designed to develop models of physiological barriers and to ensure an in vivo-like environment under static or dynamic flow conditions. Each compartment (the large upper compartment and the two lower ones) has 1 flow inlet and 1 flow outlet for the perfusion of culture media and testing solutions in both the apical and basolateral compartments.

The entire body of the chip is made of Topas, a cyclic olefin copolymer (COC) to overcome the limitations of standard PDMS. Topas does not unspecifically absorb medium contents and has high stability and ideal optical characteristics for the bright field as well as fluorescence microscopy. Topas is frequently used in medical devices due to its proven biocompatibility.

The chip comes with an 8 µm porous membrane.

Features

  • Mini Luer connection molded with the chip: leak-free
  • Highly resistant and optically clear material: Topas (COC, cyclic olefin copolymer) has high transparency (equivalent to glass) and very low grade of auto-fluorescence
  • Standard microscope glass slide size (75.5 mm x 25.5 mm x 1.5 mm)
  • Cover lid thickness: 140 µm
  • 8 µm porous PET membrane

Applications

The upper and the lower compartments are separated by the porous membrane, and they can be perfused with different culture media. A tissue interface can be created to mimic alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc (see the Documentation tab). Tissues inside the chip can be easily observed by microscopy.

Cell culture is just one potential application area of this versatile chip. The design indeed allows other different experiments such as small molecule transfer measurements, on-chip dialysis, and many more.


1x Cross-flow membrane organ-on-a-chip with chamber interaction

Please use this information to precisely define the key parameters of your cell seeding and experimental protocols.


Upper compartment Bottom compartment
Volume (µL) 281.4
101.8
Total surface (mm²) 1138
418
Ground surface (mm²) 456
185
Area of membrane interaction (mm²) 71.5
Pore size (µm) 8
Lid thickness (µm) 140

 

Porous membrane
Area of interaction (mm²) 71.5
Pore size (µm) 0.4 8
Membrane reference mcs-membrane 132 mcs-membrane 120
Pore density (pores/cm²) 4×10^6 1×10^5 +/- 0.02
Thickness (µm) 12 11.5 +/- 1.5
Material Hydrophilized polyethylene terephthalate (PET)
Color Transparent
Pore orientation Parallel, perpendicular to the surface
Imaging Yes
Chip material Topas (COC)
Lid thickness (µm) 140

Dimensions of the cross-flow membrane organ-on-a-chip with interaction chamber

Click to read more information about ChipShop chips material properties.

📄 General handling guide for cross-flow membrane chips

📄 Cross-flow membrane chip with chamber interaction Fluidic 747 datasheet

Maurer, M., Gresnigt, M. S., Last, A., Wollny, T., Berlinghof, F., Pospich, R., ... & Mosig, A. S. (2019). A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies. Biomaterials,220, 119396. https://doi.org/10.1016/j.biomaterials.2019.119396

Raasch, M., Rennert, K., Jahn, T., Peters, S., Henkel, T., Huber, O., ... & Mosig, A. (2015). Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions. Biofabrication, 7(1), 015013. doi:10.1088/1758-5090/7/1/015013

Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A. M., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., Peters, F. T., Lupp, A., Gärtner, C., Mayr, T., Bauer, M., Huber, O., & Mosig, A. S. (2015). A microfluidically perfused three dimensional human liver model. Biomaterials, 71, 119–131. https://doi.org/10.1016/j.biomaterials.2015.08.043

Payment Terms

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Worldwide shipping

We ship worldwide from our warehouse in France.

Shipping charges are calculated based on the shipping country, and can be estimated from the cart page. Our shipping rates and times are:

  • France: GLS (1-2 business days), free shipping above 350€
  • Germany, Austria, Belgium, Denmark, Spain, Hungary, Italy, Luxembourg, Netherlands, Poland, Portugal, Czechia, Slovakia:
    • GLS (2-4 business days), free shipping above 350€
    • DHL Express (1-2 business days), reduced rate above 350€
  • Bulgaria, Croatia, Estonia, Finland, Ireland, Latvia, Lithuania, Romania, Slovenia, Sweden, Greece, Cyprus, Malta: DHL Express (1-2 business days), free shipping above 400€
  • United Kingdom: DHL Express (1-2 business days), free shipping above 400€
  • USA, Canada: DHL Express (1-3 business days), free shipping above 400€
  • Switzerland: DHL Express (1-2 days), free shipping above 450€
  • Other countries: DHL Express, shipping rates based on the destination, weight and dimensions

 

If you wish to use your own shipping account, we will charge a flat fee of 15€.

We reserve the right to apply a surcharge for parcels > 30 kg, palletized shipments, or packages with dimensions exceeding the maximum dimensions of our carriers.

Please note that our standard shipping incoterms are DAP (Delivered At Place), or EXW if using your own carrier.

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