Organ-on-a-Chip - Cross-flow membrane w/ Chamber Interaction - Mini Luer
- Product SKU: CS-10001557
- Category: Microfluidic Chips, Microfluidic ChipShop, Microfluidic Organ-on-a-chip, New Products, Organ-on-a-chip Chips
This convenient and affordable Organ-on-a-Chip is a compact solution to recreate biological barriers in vitro. One chip hosts two culture chambers that are connected together through their upper compartments. Each chamber has two compartments divided by a permeable membrane.
The chip is fabricated in Topas (COC, cyclic olefin copolymer) for better light transmittance. The inlets and outlets with integrated Mini Luer connections ensure leak-free junctions with the tubing and allow experiments under dynamic flow conditions.
This Organ-on-a-Chip with a cross-flow membrane allows you to reliably mimic physiological conditions in vitro and study tissue organization, cell-cell interactions, barrier penetration, and physiological responses in a more in vivo-like environment.
Two connected culture chambers are available on the same chip, to perform two experiments in parallel or in series (body-on-a-chip) through the upper compartment connection.
This chip is designed to develop models of physiological barriers and to ensure an in vivo-like environment under static or dynamic flow conditions. Each compartment (the large upper compartment and the two lower ones) has 1 flow inlet and 1 flow outlet for the perfusion of culture media and testing solutions in both the apical and basolateral compartments.
The entire body of the chip is made of Topas, a cyclic olefin copolymer (COC) to overcome the limitations of standard PDMS. Topas does not unspecifically absorb medium contents and has high stability and ideal optical characteristics for the bright field as well as fluorescence microscopy. Topas is frequently used in medical devices due to its proven biocompatibility.
The chip comes with an 8 µm porous membrane.
Features
- Mini Luer connection molded with the chip: leak-free
- Highly resistant and optically clear material: Topas (COC, cyclic olefin copolymer) has high transparency (equivalent to glass) and very low grade of auto-fluorescence
- Standard microscope glass slide size (75.5 mm x 25.5 mm x 1.5 mm)
- Cover lid thickness: 140 µm
-
8 µm porous PET membrane
Applications
The upper and the lower compartments are separated by the porous membrane, and they can be perfused with different culture media. A tissue interface can be created to mimic alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc (see the Documentation tab). Tissues inside the chip can be easily observed by microscopy.
Cell culture is just one potential application area of this versatile chip. The design indeed allows other different experiments such as small molecule transfer measurements, on-chip dialysis, and many more.
1x Cross-flow membrane organ-on-a-chip with chamber interaction
Please use this information to precisely define the key parameters of your cell seeding and experimental protocols.
| Upper compartment |
Bottom compartment |
|
| Volume (µL) | 281.4 |
101.8 |
| Total surface (mm²) | 1138 |
418 |
| Ground surface (mm²) | 456 |
185 |
| Area of membrane interaction (mm²) | 71.5 |
|
| Pore size (µm) | 8 | |
| Lid thickness (µm) | 140 |
|
| Porous membrane | ||
| Area of interaction (mm²) | 71.5 | |
| Pore size (µm) | 0.4 | 8 |
| Pore density (pores/cm²) | 4×10^6 | 1×10^5 +/- 0.02 |
| Thickness (µm) | 12 | 11.5 +/- 1.5 |
| Material | Polyethylene terephthalate (PET) | |
| Color | Transparent | |
| Pore orientation | Parallel, perpendicular to the surface | |
Click to read more information about ChipShop chips material properties.
📄 General handling guide for cross-flow membrane chips
📄 Cross-flow membrane chip with chamber interaction datasheet
Maurer, M., Gresnigt, M. S., Last, A., Wollny, T., Berlinghof, F., Pospich, R., ... & Mosig, A. S. (2019). A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies. Biomaterials,220, 119396. https://doi.org/10.1016/j.biomaterials.2019.119396
Raasch, M., Rennert, K., Jahn, T., Peters, S., Henkel, T., Huber, O., ... & Mosig, A. (2015). Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions. Biofabrication, 7(1), 015013. doi:10.1088/1758-5090/7/1/015013
Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A. M., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., Peters, F. T., Lupp, A., Gärtner, C., Mayr, T., Bauer, M., Huber, O., & Mosig, A. S. (2015). A microfluidically perfused three dimensional human liver model. Biomaterials, 71, 119–131. https://doi.org/10.1016/j.biomaterials.2015.08.043
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