Organ-on-a-Chip - Cross-flow membrane w/ Large Chamber - Mini Luer

Organ-on-a-Chip - Cross-flow membrane w/ Large Chamber - Mini Luer

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microfluidic ChipShop

Organ-on-a-Chip - Cross-flow membrane w/ Large Chamber - Mini Luer

79.50€

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General
Content
Specifications
Documentation

General

Whereas standard ChipShop connectors only accept soft-walled tubing, this set of connectors allows connecting any 1/16" OD rigid tubing to a ChipShop chip.

Suitable for rigid tubing (PTFE, PEEK, etc.) with an OD of 1/16" (1.6 mm). Manufactured in blue TPE, max. pressure of 3.2 bar with a 1/16" OD PEEK tubing.

Plugs to block unused ports are also available in our shop.

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We recommend contacting your local customs office for detailed information on these charges if you're ordering from outside the EU, as they can vary depending on the destination and the value of your order. Please note that these additional costs are not included in the shipping fees charged by Darwin Microfluidics.

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Please remember, these charges are the responsibility of the recipient and are not included in the shipping costs paid to Darwin Microfluidics.

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The Fluidic 846 Organ-on-a-Chip by Microfluidic ChipShop offers an affordable and compact way to recreate biological barriers in vitro. Each chip features a large chamber divided by a permeable membrane, enabling co-culture within the space.

The chip is fabricated in Topas (COC, cyclic olefin copolymer) for better light transmittance. The inlets and outlets with integrated Mini Luer connections ensure leak-free junctions with the tubing and allow experiments under dynamic flow conditions.

General

This Organ-on-a-Chip with a cross-flow membrane allows you to reliably mimic physiological conditions in vitro and study tissue organization, cell-cell interactions, barrier penetration, and physiological responses in a more in vivo-like environment.

This chamber is designed to develop models of physiological barriers and to ensure an in vivo-like environment under static or dynamic flow conditions. It has 2 flow inlets and 2 outlets for the perfusion of culture media and testing solutions in both the apical and basolateral compartments.

The entire body of the chip is made of Topas, a cyclic olefin copolymer (COC) to overcome the limitations of standard PDMS. Topas does not unspecifically absorb medium contents and has high stability and ideal optical characteristics for the bright field as well as fluorescence microscopy. Topas is frequently used in medical devices due to its proven biocompatibility.

The chip comes with an 8 µm porous membrane.

Features

Mini Luer connection molded with the chip: leak-free
Highly resistant and optically clear material: Topas (COC, cyclic olefin copolymer) has high transparency (equivalent to glass) and very low grade of auto-fluorescence
Standard microscope glass slide size (75.5 mm x 25.5 mm x 1.5 mm)
Cover lid thickness: 140 µm
8 µm porous PET membrane

Cross-flow porous membrane	Upper/Apical compartment	Bottom/Basolateral compartment

Applications and examples from published papers

The upper and the lower compartments are separated by the porous membrane, and they can be perfused with different culture media. A tissue interface can be created to mimic alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc. Tissues inside the chip can be easily observed by microscopy.

Cell culture is just one potential application area of this versatile chip. The design indeed allows other different experiments such as small molecule transfer measurements, on-chip dialysis, and many more.

Intestine-on-chip

Maurer M. et al., A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies, Biomaterials 2019 (Download)

3D microphysiological model of the human intestine.

In this work, the model displays the physiological immune tolerance of the intestinal lumen to microbial-associated molecular patterns and can, therefore, be colonized with living microorganisms. The authors demonstrate that microbial interactions can be efficiently investigated using this chip creating a more physiological and immunocompetent microenvironment.

Endothelial Barrier-on-chip

Raasch M. et al., Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions, Biofabrication 2015, 7: 015013 (Download)

The authors investigated cell viability, expression of endothelial markers, and cell adhesion molecules of ECs dynamically cultured under low and high shear stress. This chip allows an effective supply with nutrition medium, discharge of catabolic cell metabolites, and defined application of shear stress to ECs under laminar flow conditions.

They show that ECs cultured in the chip form a tight EC monolayer with increased cellular density and enhanced cell layer thickness compared to static and two-dimensionally perfused cell culture conditions. Endothelial layers in the chip express higher amounts of EC marker proteins von-Willebrand-factor and PECAM-1.

Liver-on-chip

Rennert K. et al., A microfluidically perfused three dimensional human liver model, Biomaterials 2015, 119-131 (Download)

Liver-on-chip images from the linked article

The microfluidically perfused chip enables sufficient nutrition supply and resembles morphological aspects of the human liver sinusoid. It utilizes a suspended membrane as a cell substrate mimicking the space of Disse and the perfusion enhances the formation of hepatocyte microvilli. The authors stated that the perfused liver chip shares relevant morphological and functional characteristics with the human liver and represents a new in vitro research tool to study human hepatocellular physiology at the cellular level under conditions close to the physiological situation.

During culture in the biochip HepaRG cells consistently differentiate into cells exhibiting a hepatocyte phenotype and into cells with biliary epithelial cell phenotype that self-organize into a hepatocyte layer with functional bile ducts.

Content

1x Cross-flow membrane organ-on-a-chip with a large chamber

Specifications

Please use this information to precisely define the key parameters of your cell seeding and experimental protocols.

	Upper compartment	Bottom compartment
Volume (µL)	500	402.8
Total surface (mm²)	1795.5	1818.7
Ground surface (mm²)	803	674.7
Lid thickness (µm)	140

	Porous membrane
Area of interaction (mm²)	395.5
Pore size (µm)	8
Membrane reference	mcs-membrane 120
Pore density (pores/cm²)	1×10^5 +/- 0.02
Thickness (µm)	11.5 +/- 1.5
Material	Hydrophilized polyethylene terephthalate (PET)
Color	Transparent
Pore orientation	Parallel, perpendicular to the surface
Imaging	Yes
Chip material	Topas (COC)
Lid thickness (µm)	140

Dimensions of the cross-flow membrane organ-on-a-chip with a large chamber

Documentation

Click to read more information about ChipShop chips material properties.

? General handling guide for cross-flow membrane chips (available soon)

? Cross-flow membrane chip with large chamber Fluidic 846 datasheet

Maurer, M., Gresnigt, M. S., Last, A., Wollny, T., Berlinghof, F., Pospich, R., ... & Mosig, A. S. (2019). A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies. Biomaterials,220, 119396. https://doi.org/10.1016/j.biomaterials.2019.119396

Raasch, M., Rennert, K., Jahn, T., Peters, S., Henkel, T., Huber, O., ... & Mosig, A. (2015). Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions. Biofabrication, 7(1), 015013. doi:10.1088/1758-5090/7/1/015013

Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A. M., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., Peters, F. T., Lupp, A., Gärtner, C., Mayr, T., Bauer, M., Huber, O., & Mosig, A. S. (2015). A microfluidically perfused three dimensional human liver model. Biomaterials, 71, 119–131. https://doi.org/10.1016/j.biomaterials.2015.08.043

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Organ-on-a-Chip - Cross-flow membrane w/ Large Chamber - Mini Luer

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