SynRAM 3D Inflammation Model

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Product SKU: SY-401002
Category: Microfluidic Chips, Microfluidic Organ-on-a-chip, Organ-on-a-chip Bundles, SynVivo

SynRAM model is a complete platform to study the inflammation pathway in a realistic environment. Enabling real-time tracking of rolling, adhesion and migration processes, it recreates a histological slice of co-cultured tissue and/or tumor cells with a lumen of endothelial cells.

Chip only, or available in two kit sizes to start using the setup out of the box!

Average lead time: 3-4 weeks

€315.00

SynRAM model is a complete platform to study the inflammation pathway in a realistic environment. Enabling real-time tracking of rolling, adhesion and migration processes, it recreates a histological slice of co-cultured tissue and/or tumor cells with a lumen of endothelial cells.

Chip only, or available in two kit sizes to start using the setup out of the box!

SynVivo's SynRAM model is designed to overcome the limitations of devices classically used in biology by taking into account fluid shear and size/topology observed in vivo.

The device allows to recreate the microenvironment and to easily model the transmigration phenomenon. By recapitulating a histological slice of co-cultured tissue and/or tumor cells with an endothelial cell lumen, the SynVivo platform provides a physiologically realistic model while allowing real-time monitoring of the rolling, adhesion, and migration processes.

Mimic the physiological shear stress in a microvascular environment
In vivo-like vascular morphology with fully enclosed lumen
Cell-to-cell interaction is possible with co-culture
Get real-time quantitative rolling, adhesion, and migration

This assay has been successfully validated against in vivo studies showing an excellent correlation with rolling speeds, adhesion patterns, and migration processes.

To run SynRAM assays, two kits formats are available:

Starter Kit	Assay Kit
10 SynRAM chips (choice of IMN2 radial or SMN2 microvascular network chips) Accessories including tubing, clamps, needles and syringes Pneumatic priming device (required for priming tubing to remove air)	10 SynRAM chips (choice of IMN2 radial or SMN2 microvascular network chips) Accessories including tubing, clamps, needles and syringes

To only order the chip, choose "Chip only" in the kit selection.

Examples of models developed using SynRAM

SynRAM rolling, adhesion and migration

Real-time visualization of rolling, adhesion and migration

Real-time tracking of single-cell adhesion with SynRAM

Real-time tracking of single-cell migration

Starter Kit	Assay Kit	Chip only
10 SynRAM chips (choice of IMN2 radial or SMN2 microvascular network chips) Accessories including tubing, clamps, needles and syringes Pneumatic priming device (required for priming tubing to remove air)	10 SynRAM chips (choice of IMN2 radial or SMN2 microvascular network chips) Accessories including tubing, clamps, needles and syringes	3x SynRAM chip (choice of IMN2 radial or SMN2 microvascular network chips)

IMN2 radial

SMN2 co-culture microvascular network

Outer channel (OC): 200 μm
Tissue chamber: 1.8 mm diameter
Depth: 100 μm
Pillar spacing (SP): 8 µm
Travel (T): 50 µm
Pillar height: 100 µm

Idealized co-culture chip microvascular network with pillar barrier.

Pillar height: 3 µm
Pillar diameter: 10 µm
Separation: 50 µm
Depth: 100 µm

📄SynVivo brochure

📄SynRAM idealized network technical manual

📄SynRAM microvascular network technical manual

📄SynRAM starter kit contents & description

📄SynRAM assay kit contents & description

Soroush, F., Tang, Y., Mustafa, O., Sun, S., Yang, Q., Kilpatrick, L. E., & Kiani, M. F. (2020). Neutrophil‐endothelial interactions of murine cells is not a good predictor of their interactions in human cells. The FASEB Journal, 34(2), 2691-2702. https://doi.org/10.1096/fj.201900048R

Yang, Q., Langston, J. C., Tang, Y., Kiani, M. F., & Kilpatrick, L. E. (2019). The role of tyrosine phosphorylation of protein kinase C delta in infection and inflammation. International journal of molecular sciences, 20(6), 1498. https://doi.org/10.3390/ijms20061498

Soroush, F., Tang, Y., Zaidi, H. M., Sheffield, J. B., Kilpatrick, L. E., & Kiani, M. F. (2018). PKCδ inhibition as a novel medical countermeasure for radiation‐induced vascular damage. The FASEB Journal, 32(12), 6436-6444. https://doi.org/10.1096/fj.201701099

Soroush, F., Zhang, T., King, D. J., Tang, Y., Deosarkar, S., Prabhakarpandian, B., ... & Kiani, M. F. (2016). A novel microfluidic assay reveals a key role for protein kinase C δ in regulating human neutrophil–endothelium interaction. Journal of Leukocyte Biology, 100(5), 1027-1035. https://doi.org/10.1189/jlb.3MA0216-087R

Lamberti, G., Soroush, F., Smith, A., Kiani, M. F., Prabhakarpandian, B., & Pant, K. (2015). Adhesion patterns in the microvasculature are dependent on bifurcation angle. Microvascular research, 99, 19-25. https://doi.org/10.1016/j.mvr.2015.02.004

Lamberti, G., Prabhakarpandian, B., Garson, C., Smith, A., Pant, K., Wang, B., & Kiani, M. F. (2014). Bioinspired microfluidic assay for in vitro modeling of leukocyte–endothelium interactions. Analytical chemistry, 86(16), 8344-8351. https://doi.org/10.1021/ac5018716

Lamberti, G., Tang, Y., Prabhakarpandian, B., Wang, Y., Pant, K., Kiani, M. F., & Wang, B. (2013). Adhesive interaction of functionalized particles and endothelium in idealized microvascular networks. Microvascular research, 89, 107-114. https://doi.org/10.1016/j.mvr.2013.03.007

Tousi, N., Wang, B., Pant, K., Kiani, M. F., & Prabhakarpandian, B. (2010). Preferential adhesion of leukocytes near bifurcations is endothelium independent. Microvascular research, 80(3), 384-388. https://doi.org/10.1016/j.mvr.2010.07.001

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France: GLS (1-2 business days), free shipping above 350€
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