This sorting chip is designed for the high-throughput analysis and sorting of single cells in droplets. Droplets are first re-injected in one of the inlets and spaced by a flow focusing design. It is followed by an active sorting site with electrodes, to be used with a high-voltage amplifier or Atrandi Biosciences Styx instrument.
1 device per chip, with 1 mm punched holes.
General
This droplet sorting chip in PDMS combines a standard flow focusing design for the re-injection and spacing of the droplets, and an active droplet sorter. Droplets should be generated in a first chip before being re-injected.
Electrical connection from the sorting site to a high-voltage amplifier is made with ITO electrodes and couplers.
Fluidic channels are coated with a silane mix after bonding to guarantee good and consistent hydrophobic properties.
Content
1x Active droplet sorting chip in PDMS
Specifications
| |
DG-MCY-R1 |
| Nozzle width |
50 µm |
| Collection channel width |
200 µm |
| Channel height |
30 µm |
| Material |
PDMS |
| Dimensions |
standard glass slide (75 x 25 mm) |
| Number of devices per chip |
1 |
| Number of inlets |
2 |
| Number of outlets |
2 |
| Punched hole size |
1 mm |
| For rigid tubing OD |
1.06 mm |
| Couplers for soft-walled tubing |
18G |
| Electrode Type |
Etched |
Documentation
Mazutis, L., Gilbert, J.R., Ung, W.L., Weitz, D.A., Griffiths, A.D., & Heyman, J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature Protocols, 8, 870-891. https://doi.org/10.1038/nprot.2013.046
