This microfluidic chip uses four liquid channels to deliver cells barcoding hydrogel beads, reagents, and oil to create a barcoded emulsion. 6 devices per chip, with 0.5 mm, 1 mm, or 1.2 mm punched holes to accommodate most tubing sizes.

The design enables you to generate droplets from 80 µm to 120 µm.

General

This microfluidic chip in PDMS can be used for single-cell transcriptomics experiments. Each chip has 6 microfluidic devices, with 4 inlets and 1 outlet each, in a standard microscope slide format. They enable you to generate droplets from 80 µm to 120 µm.

Coated with a silane mix after bonding to guarantee good and consistent hydrophobic properties.

• Material: PDMS
• Dimensions: standard glass slide (75 x 25 mm)
• Number of devices per chip: 6
• Inlets: 4
• Outlet: 1
• Punched hole size: 1 mm (some 0.5 and 1.2 punch sizes are still available)

Choose the punched hole size according to your requirements:

• 0.5 mm: for 1/32" OD rigid tubing (PTFE, PEEK, FEP, PFA, etc.) or 23G couplers for soft-walled tubing
• 1.0 mm: for 1.06 mm OD rigid tubing (PTFE, PEEK, FEP, PFA, etc.) or 18G couplers for soft-walled tubing
• 1.2 mm: for 1/16" OD rigid tubing (PTFE, PEEK, FEP, PFA, etc.) or 18G couplers for soft-walled tubing

Content

1x Cell barcoding chip in PDMS

Specifications

Documentation

Zilionis, R., Nainys, J., Veres, A., Savova, V., Zemmour, D., Klein, A. M., & Mazutis, L. (2017). Single-cell barcoding and sequencing using droplet microfluidics. Nature protocols, 12(1), 44–73. https://doi.org/10.1038/nprot.2016.154